Abstract:

The authors investigated the bioaugmentation performances of a selected bacterial strain for denitrification in a packed bed bioreactor using PBS as solid carbon source. Fluorescence quantitative PCR was employed to analyze the microbial community composition in the bioreactor. Results indicated that the denitrifier named W14 could remarkably improve denitrification efficiency. At HRT of 0.5 h, nitrogen removal was found to be higher than 90% and the residual DOC of effluent was greatly reduced. Addition of selected denitrifiers led to an increase of nirS gene abundance and proportion, indicating that inoculation of selected bacterial strain could boost denitrifiers’ proliferation and enhance nitrogen removal eventually.

Key words: denitrifier, bioaugmentation, nitrogen removal, solid carbon source, packed bed bioreactor, fluorescence quantitative PCR

摘要：

以聚丁二酸丁二醇酯(PBS)作为固相可生物降解模式碳源的生物填充床, 针对分离获得的高效反硝化菌开展强化生物脱氮的特性研究, 并利用荧光定量PCR解析反应器的微生物群落结构。结果表明, 投加反硝化菌(W14)可以明显地提高反硝化脱氮效率, 当水力停留时间(HRT)为0.5 h时, 反硝化菌强化脱氮生物反应器的脱氮效率达到90%以上, 且能有效地降低出水残余的DOC浓度。荧光定量PCR结果表明, 高效反硝化菌投加强化能够增加nirS基因丰度和比例, 较好地解释了不同接种生物反应器的脱氮效果差异, 即反硝化菌的强化作用能有效增加反硝化菌数量并强化脱氮效果。

关键词: 反硝化菌, 生物强化, 脱氮, 固相碳源, 生物填充床, 荧光定量PCR