Acta Scientiarum Naturalium Universitatis Pekinensis ›› 2020, Vol. 56 ›› Issue (4): 772-776.DOI: 10.13209/j.0479-8023.2020.046

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Variations of 4 Antibiotic Resistance Genes in a Sewage Treatment Plant

BAO Yingyu1, XIE Hui2, CHEN Lüjun2, WEN Donghui1,†   

  1. 1. College of Environmental Sciences and Engineering, Peking University, Beijing 100871 2. School of Environment, Tsinghua University, Beijing 100084
  • Received:2019-06-13 Revised:2019-10-31 Online:2020-07-20 Published:2020-07-20
  • Contact: WEN Donghui, E-mail: dhwen(at)


包樱钰1, 谢辉2, 陈吕军2, 温东辉1,†   

  1. 1. 北京大学环境科学与工程学院, 北京 100871 2. 清华大学环境学院, 北京 100084
  • 通讯作者: 温东辉, E-mail: dhwen(at)
  • 基金资助:
    国家自然科学基金(51678003, 51678334)资助


In order to understand the variations of antibiotic resistance genes (ARGs) in sewage treatment plants (STPs), the distribution and removal efficiencies of 4 subtypes of intracellular ARGs (sulII, ermB, tetC and blaPSE-1) and class I integron integrase gene (intI1) in a middle-scale STP in Hebei Province were detected by PCR and real-time fluorescent quantitative PCR (qPCR). 4 ARGs and intI1 were found in all water samples and 1.26–2.30 orders of magnitude of ARGs were removed by the STP. Correlation analysis showed that intI1 and water quality factors including pH, COD, and NH3-N might affect the distribution and diffusion of tetC, ermB, and blaPSE-1. The final effluent of a STP may promote the spread of ARGs in surface water system.

Key words: antibiotic resistance genes (ARGs), sewage treatment plants (STPs), A2/O process, real-time fluorescence quantitative PCR (qPCR)


为探究抗生素抗性基因(ARGs)在城镇污水处理系统中的沿程变化, 针对4种关注度较高的ARGs (sulII, ermB, tetCblaPSE-1)和I型整合子的整合酶基因(intI1), 选取京津冀地区一座中小城镇的污水处理厂, 使用普通PCR和实时荧光定量PCR技术, 对各工艺单元的水样进行细胞内ARGs和 intI1的定性及定量检测分析。结果表明, 4种ARGs和intI1普遍存在于城镇污水处理系统中, 出水中ARGs和intI1的绝对丰度降低, 去除量在 1.26~2.30 logs之间。相关性分析表明, intI1和水质因子(pH, COD和NH3-N)可能与4种ARGs的传播扩散有关。

关键词: 抗生素抗性基因(ARGs), 城镇污水处理系统, A2/O工艺, 实时荧光定量PCR