Abstract:

Analysis of exhaled VOCs from health and patients may help building association between VOCs and different diseases condition, which could offer a possibility of noninvasive monitoring of disease. VOCs were collected in 30 healthy subjects and 60 patients with upper respiratory tract infection. Analysis of 97 VOCs was
performed by gas chromatography and mass spectrometry (GC-MS). The concentration of alkanes, alkenes, halogenated hydrocarbon, oxygenated hydrocarbons and aromatic hydrocarbons in health and patients breath was analyzed. Analysis of variance (ANOVA), principal component analysis (PCA) were used to analyze the VOC differences from patients and healthy ones. The results show that the concentration of isoprene differ among patients, healthy ones and indoor air (P<0.05). Patients show higher concentration of n-pentane compared to healthy ones (P<0.05). Patients with bacterial upper respiratory tract infection show higher concentration of propanal compared to healthy ones. The results of PCA show that there were significant VOC differences between patients with upper respiratory tract infection and healthy ones (P=0.019), but no differences between bacterial and non-bacterial upper respiratory tract infection.

Key words: exhaled VOCs, upper respiratory tract infection, healthy, ANOVA-PCA

摘要：

研究健康人与病患呼出气VOCs成分的异同, 为上呼吸道感染分型的无创快速诊断提供信息。采集60例上呼吸道感染患者、30例健康人呼出气, 利用GC-MS定量检测97种VOCs, 分析健康人及病患呼出气中烷烃、烯烃、卤代烃、含氧烃、芳香烃等物质的分布特点, 并利用ANOVA-PCA, 分析室内空气以及健康人和病患呼出气中VOCs的异同。结果表明, 异戊二烯浓度在病患、健康人呼出气及室内空气中均有显著差异(P<0.05), 且呈现病人>健康人>室内空气的特点; 正戊烷浓度在病患组显著高于健康人组(P<0.05); 丙醛浓度在细菌性上感组显著高于健康组(P=0.019)。PCA分析结果显示, 健康人与上呼吸道感染病患呼出气差异明显, 不同类型病患呼出气VOCs没有明显差异。

关键词: 呼出气VOCs, 上呼吸道感染, 健康人, ANOVA-PCA