北京大学学报自然科学版 ›› 2021, Vol. 57 ›› Issue (5): 894-902.DOI: 10.13209/j.0479-8023.2021.066

上一篇    下一篇

微塑料和全氟辛烷磺酸类物质共暴露对翡翠贻贝滤食率和抗氧化系统的影响

徐澎, Muhammad Junaid, 刘燕, 陈裕鹏, 毕春晴, 许楠†   

  1. 深圳市重金属污染控制与资源化重点实验室, 北京大学深圳研究生院环境与能源学院, 深圳 518055
  • 收稿日期:2020-06-11 修回日期:2020-07-03 出版日期:2021-09-20 发布日期:2021-09-20
  • 通讯作者: 许楠, E-mail: xunan(at)pkusz.edu.cn
  • 基金资助:
    国家自然科学基金(51879003, 51939009)资助

Effects of Microplastics and PFOS Co-exposure on the Filtration Rate and Antioxidant System of Perna viridis

XU Peng, Muhammad Junaid, LIU Yan, CHEN Yupeng, BI Chunqing, XU Nan†   

  1. Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055
  • Received:2020-06-11 Revised:2020-07-03 Online:2021-09-20 Published:2021-09-20
  • Contact: XU Nan, E-mail: xunan(at)pkusz.edu.cn

PDF

摘要：

为了探索微塑料和全氟辛烷磺酸类物质(PFOS)在海洋生物体内的复合污染毒性机制, 以翡翠贻贝为研究对象, 在聚苯乙烯微塑料(0.2 μm) 4.55×108个/L以及PFOS浓度为10, 100和1000 μg/L的条件下, 研究PFOS或微塑料单独暴露以及二者复合暴露对翡翠贻贝滤食率、活性氧水平(ROS)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、谷胱甘肽巯基转移酶(GST)活性和谷胱甘肽还原酶(GR)活性的影响。结果表明, 与溶剂对照组相比, 暴露于微塑料和PFOS后翡翠贻贝的滤食率均没有显著变化。在翡翠贻贝的鳃、内脏团和性腺中, 微塑料或PFOS的胁迫都会引起酶响应的显著变化。与PFOS单独暴露组相比, 微塑料和 1000 μg/L PFOS共同暴露时, ROS水平在鳃和性腺中显著提升, 在内脏团中显著下降; MDA含量在鳃和内脏团中显著提升, 在性腺中显著下降; GST和GR活性在鳃中显著下降, 在性腺中显著提高。研究结果说明微塑料会改变翡翠贻贝对PFOS的氧化应激响应。

关键词: PFOS, 海洋微塑料, 共暴露, 氧化应激, 滤食率, 翡翠贻贝

Abstract:

In order to explore the composite pollution toxicity mechanism of microplastics and perfluorooctane sulfonate (PFOS) in Perna viridis, the effects of PFOS (10, 100, 1000 μg/L) and microplastics (0.2 μm, 4.55×108 numbers/L) were studied under single- or co-exposure on the filtration rate, reactive oxygen level (ROS), malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione S-transferase (GST) activity and gluathione reductase (GR) activity. The results showed that the filtration rate of mussels after exposure to microplastics and PFOS did not change significantly compared to the solvent control. In gills, visceral mass and gonads of mussels, the stress of microplastics and PFOS could cause significant changes in enzyme response. After co-exposure of microplastics and 1000 μg/L PFOS, compared with single-PFOS exposure groups, ROS level significantly increased in gills and gonads, whereas decreased in visceral mass; MDA content increased in gills and visceral mass, whereas decreased in gonads; GST and GR activity was significantly reduced in gills, whereas increased in gonads. The results indicated that microplastics could change the oxidative stress response of mussels to PFOS.

Key words: PFOS, marine microplastics, co-exposure, oxidative stress, filtration rate; Perna viridis

版权所有 © 《北京大学学报（自然科学版）》编辑部
技术支持：北京玛格泰克科技发展有限公司