Treatment of 2,4-DNT-3-SA Contaminated Soil by a Biological Leaching Method

doi:10.13209/j.0479-8023.2017.094

Abstract

Abstract:

A static biological leaching method was used to treat the 2,4-DNT-3-SA contaminated soil. The effects of oxygen conditions, soil to water ratio and temperature on the removal efficiency of 2,4-DNT-3-SA were evaluated. The result suggested that under the aerobic and 37ºC conditions, the removal efficiency of 2,4-DNT-3-SA reached more than 98% when the ratio of soil to water was 10:5. High-throughput sequencing results indicated that the dominant bacteria in the soil were Genus Pseudomonas, Sphingobium and Sporolactobacillus.

Key words: biological leaching, 2,4-DNT-3-SA, soil, high-throughput sequencing

摘要：

采用静态生物淋洗法处理含 2,4-二硝基甲苯-3-磺酸钠(2,4-DNT-3-SA)的土壤。考察供氧条件、土水比及温度对2,4-DNT-3-SA去除效果的影响, 结果表明, 在好氧、37ºC、土水比为10:5的条件下, 2,4-DNT-3-SA去除率最高, 达到 98.75%。采用高通量测序法分析土壤中微生物菌落结构及多样性, 结果表明, 土壤中的优势菌属主要为假单胞菌属、鞘脂菌属和芽孢乳杆菌属。

关键词: 生物淋洗, 2,4-DNT-3-SA, 土壤, 高通量测序

Tianyu ZHANG, Quanlin ZHAO, Zhenzhong ZHANG, Zhenming JIANG, Hongping SU, Xuewen GAO, Zhengfang YE. Treatment of 2,4-DNT-3-SA Contaminated Soil by a Biological Leaching Method[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2017, 53(5): 899-904.

张天宇, 赵泉林, 张振中, 姜振明, 苏宏平, 高学文, 叶正芳. 生物淋洗法修复2,4-DNT-3-SA污染土壤[J]. 北京大学学报自然科学版, 2017, 53(5): 899-904.

Figures/Tables 8

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门	相对丰度/%
门	A1	A2	A3	A4	B1	B2	B3	B4
Proteobacteria	60.64	78.03	34.95	40.39	56.64	38.64	87.65	27.86
Firmicutes	26.83	15.50	28.09	46.81	35.74	36.93	5.81	59.32
Actinobacteria	8.68	3.11	29.87	9.14	4.77	18.98	5.24	9.86
Bacteroidetes	3.10	2.04	0.78	1.82	1.70	4.21	0.27	1.95
Chloroflexi	0.11	0.22	4.29	0.67	0.46	0.56	0.25	0.44
Gemmatimonadetes	0.02	0.09	0.61	0.27	0.06	0.09	0.13	0.07
Verrucomicrobia	0.01	0.41	0	0.09	0	0	0.39	0.01
Cyanobacteria	0.02	0	0	0.24	0.06	0.08	0.01	0
Thermotogae	0	0	0.22	0	0.03	0	0	0.12
Crenarchaeota	0	0	0	0	0	0	0.07	0

门	相对丰度/%
门	A1	A2	A3	A4	B1	B2	B3	B4
Proteobacteria	60.64	78.03	34.95	40.39	56.64	38.64	87.65	27.86
Firmicutes	26.83	15.50	28.09	46.81	35.74	36.93	5.81	59.32
Actinobacteria	8.68	3.11	29.87	9.14	4.77	18.98	5.24	9.86
Bacteroidetes	3.10	2.04	0.78	1.82	1.70	4.21	0.27	1.95
Chloroflexi	0.11	0.22	4.29	0.67	0.46	0.56	0.25	0.44
Gemmatimonadetes	0.02	0.09	0.61	0.27	0.06	0.09	0.13	0.07
Verrucomicrobia	0.01	0.41	0	0.09	0	0	0.39	0.01
Cyanobacteria	0.02	0	0	0.24	0.06	0.08	0.01	0
Thermotogae	0	0	0.22	0	0.03	0	0	0.12
Crenarchaeota	0	0	0	0	0	0	0.07	0

属	相对丰度/%
属	A1	A2	A3	A4	B1	B2	B3	B4
Sporolactobacillus	1.88	8.96	8.45	9.67	1.51	3.71	3.80	16.1
Sphingobium	4.00	4.88	4.76	11.08	24.87	16.87	16.69	15.15
Pseudomonas	20.51	16.68	26.59	26.31	9.49	10.09	12.22	7.63
Lactococcus	7.89	5.78	2.29	0.08	4.05	4.24	5.72	0.90
Clostridium	1.82	4.15	6.15	0.27	2.16	4.00	3.02	7.29
Halomonas	0.13	0.35	0.49	0	0.09	0.85	0.60	0.49
Staphylococcus	0	0.09	0	2.62	0	0.50	0.08	3.33
Citrobacter	2.3	1.64	10.64	3.73	1.95	3.58	4.43	5.39
Cellulomonas	4.38	0.69	0.50	0.20	0.28	0.46	1.48	4.13
Azospirillum	0.46	0.39	0.23	0.76	0.70	0.88	0.34	3.12

属	相对丰度/%
属	A1	A2	A3	A4	B1	B2	B3	B4
Sporolactobacillus	1.88	8.96	8.45	9.67	1.51	3.71	3.80	16.1
Sphingobium	4.00	4.88	4.76	11.08	24.87	16.87	16.69	15.15
Pseudomonas	20.51	16.68	26.59	26.31	9.49	10.09	12.22	7.63
Lactococcus	7.89	5.78	2.29	0.08	4.05	4.24	5.72	0.90
Clostridium	1.82	4.15	6.15	0.27	2.16	4.00	3.02	7.29
Halomonas	0.13	0.35	0.49	0	0.09	0.85	0.60	0.49
Staphylococcus	0	0.09	0	2.62	0	0.50	0.08	3.33
Citrobacter	2.3	1.64	10.64	3.73	1.95	3.58	4.43	5.39
Cellulomonas	4.38	0.69	0.50	0.20	0.28	0.46	1.48	4.13
Azospirillum	0.46	0.39	0.23	0.76	0.70	0.88	0.34	3.12