Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae

Abstract

Abstract: The psaC Gene of Synechococcus sp. PCC 7002 encodes the F_A/F_B aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae.Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

Key words: Cyanobacteria, psaC, Saccharomyces cerevisiae, transformation

摘要： 聚球蓝藻(Synechococcus sp.PCC 7002)的psaC是光系统I的F_A/F_B脱辅基蛋白基因，其分子量8.9kD蛋白中含有2个4Fe-4S中心。构建了psaC基因的表达载体pYES2-psaC，将表达载体转化到酵母(Saccharomyces cerevisiae)中，Southern杂交证明psaC基因已被转化，诱导培养后的蛋白电泳显示psaC基因获得高效表达。

关键词: 蓝藻, psaC, 酵母, 转化

CLC Number:

LI Bin,LI Tao,TONG Xuemei,WANG Donghui,ZHAO Jindong,WU Guangyao. Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

李斌,李涛,童雪梅,王东辉,赵进东,吴光耀. 蓝藻光合基因psaC在酵母中的转化和表达[J]. 北京大学学报（自然科学版）.

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URL: https://xbna.pku.edu.cn/EN/

https://xbna.pku.edu.cn/EN/Y2001/V37/I6/755

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Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae

蓝藻光合基因psaC在酵母中的转化和表达

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