Acta Scientiarum Naturalium Universitatis Pekinensis

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Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae

LI Bin,LI Tao,TONG Xuemei,WANG Donghui,ZHAO Jindong,WU Guangyao   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:2000-05-18 Online:2001-11-20 Published:2001-11-20



  1. 北京大学生命科学学院,北京,100871

Abstract: The psaC Gene of Synechococcus sp. PCC 7002 encodes the FA/FB aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae.Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

Key words: Cyanobacteria, psaC, Saccharomyces cerevisiae, transformation

摘要: 聚球蓝藻(Synechococcus sp.PCC 7002)的psaC是光系统I的FA/FB脱辅基蛋白基因,其分子量8.9kD蛋白中含有2个4Fe-4S中心。构建了psaC基因的表达载体pYES2-psaC,将表达载体转化到酵母(Saccharomyces cerevisiae)中,Southern杂交证明psaC基因已被转化,诱导培养后的蛋白电泳显示psaC基因获得高效表达。

关键词: 蓝藻, psaC, 酵母, 转化

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