This paper studied the heat resistance of phycocyanin and the accumulation of phycocyanin under different light conditions in thermophilic cyanobacteria. With 30°C, 40°C, 50°C, 60°C, 65°C, 70°C, 80°C, 90°C as the experimental group, 25°C and 4°C as the control group, the placement time was set to 5 hours and 14 days. Alignments of both chain α and β were conducted to figure out the heat resistant mechanism. PKUAC-SCTE542 was cultured under different photoperiods and different combinations of light sources, and its growth and phycocyanin content were studied to explore the light conditions which were suitable for phycocyanin production. Shaking incubators with narrow band light at following wavelengths: white (400–800 nm), red (654 nm), green (511 nm), and blue (454 nm), photoperiods of 8L/16D, 12L/12D, and 16L/8D, were tested. Dry weight and content of extracted phycocyanin were measured. PKUAC-SCTE542 phycocyanin kept stable at 65°C environments, and still maintained 60% activity after being placed at 60°C for 14 days. Under the conditions of red light and photoperiod of 8L/16D, the yield of phycocyanin was the highest; while the productivity of phycocyanin was the highest when red light and photoperiod of 16L/8D were set. In result, PKUAC-SCTE542 showed great heat resistance ability in this research. With light screening done, the phycocyanin yield increased by nearly 100 mg/gDCW.

对PKUAC-SCTE542藻蓝蛋白的耐热性进行测试, 探究其热稳定性能。同时, 在不同光周期和不同光质光源组合条件下培养嗜热蓝细菌PKUAC-SCTE542, 研究其生长情况和藻蓝蛋白含量, 探索适用于藻蓝蛋白生产的光照条件。选取30°C, 40°C, 50°C, 60°C, 65°C, 70°C, 80°C, 90°C为实验组, 25°C和4°C为对照组, 放置时间设为5小时和14天, 用紫外分光光度计测定藻蓝蛋白全光谱图, 考察不同温度和放置时间对藻蓝蛋白的影响, 再将E542-PC的α链和β链与蛋白质晶体结构数据库中藻蓝蛋白进行比对, 深入了解其耐热机制。分别在光波长为白光(400~800 nm) 、红光(654 nm) 、绿光(511 nm) 和蓝光(454 nm), 光周期为8L/16D, 12L/12D和16L/8D的条件下培养蓝细菌, 测定其生物质干重以及藻蓝蛋白含量。结果表明, PKUACSCTE542藻蓝蛋白在 65°C 环境可以保持稳定, 60°C放置14天仍表现出60%的热稳定性。光质为红光、光周期为16L/8D的条件下, 藻蓝蛋白绝对产量最大; 光质为红光、光周期为8L/16D时, 藻蓝蛋白产率最高。实验结果表明PKUAC-SCTE542藻蓝蛋白的耐热性能较好, 经过光照条件筛选, 藻蓝蛋白产量可提高近100 mg/gDCW。