Acta Scientiarum Naturalium Universitatis Pekinensis ›› 2019, Vol. 55 ›› Issue (6): 1129-1140.DOI: 10.13209/j.0479-8023.2019.088

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Investigation on Pyridine-Degrading Performances and Biofilm-Forming Properties of 12 Pyridine-Degrading Bacterial Strains

ZHAO Xiaoxi1, XIONG Fuzhong1, WEN Donghui1,†, LI Qilin2   

  1. 1. College of Environmental Sciences and Engineering, Peking University, Beijing 100871 2. College of Civil and Environmental Engineering, Rice University, Texas 77005
  • Received:2018-12-13 Revised:2019-06-14 Online:2019-11-20 Published:2019-11-20
  • Contact: WEN Donghui, E-mail: dhwen(at)


赵小希1, 熊富忠1, 温东辉1,†, 李琪琳2   

  1. 1. 北京大学环境科学与工程学院, 北京 100871 2. College of Civil and Environmental Engineering, Rice University, Texas 77005
  • 通讯作者: 温东辉, E-mail: dhwen(at)
  • 基金资助:
    国家自然科学基金(51378019, 51529801)资助


Choosing pyridine as the target pollutant, the pyridine-degrading performances and biofilm-forming properties of 12 pyridine-degrading bacterial strains, isolated from a coking wastewater treatment plant, were investigated. The results show that all the 12 strains had high degradation activity of pyridine. For two efficient degrading strains, Pseudomonas sp. ZX01 and Arthrobacter sp. ZX07, the optimal temperature and pH to degrade pyridine were 35oC and 7.0, respectively. Pyridine with initial concentration ranged in 100 to 2000 mg/L could be completely degraded by either strain. Furthermore, the biofilm-forming abilities of the 12 strains were significantly different. The cellular properties of extracellular protein secretion, extracellular polysaccharide secretion, and flagella-mediated swimming motility were identified to have significant positive correlations with their biofilmforming capacities. Our study provides scientific references for constructing a degrading-biofilm system for refractory wastewater treatment.

Key words: pyridine-degrading bacteria, environmental factors, biofilm, extracellular polymeric substances


以吡啶为目标污染物, 考察从焦化废水处理系统中分离的 12 株高效吡啶降解菌对吡啶的降解性能和生物膜形成特性, 以期为在废水处理系统中构建降解型生物膜提供理论参考。结果表明: 12株菌都具有较高的吡啶降解活性, 其中代表性菌株Pseudomonas sp. ZX01和Arthrobacter sp. ZX07降解吡啶的最适温度是35oC, 最适pH是7.0, 在初始吡啶浓度为100~2000 mg/L的范围内, 降解率均达到100%。不同菌株的生物膜形成能力差异明显, 胞外蛋白分泌量、胞外多糖分泌量和由鞭毛参与的游动能力与各株菌的生物膜形成能力之间存在显著的正相关关系。

关键词: 吡啶降解菌, 环境因子, 生物膜, 胞外聚合物