Abstract:

In order to reveal the correlation between microbial diversity and the pollution degrees of the source water in a reservoir in Guangdong Province, for better understanding the ecological functions of this region, the environmental factors and microbial community were analyzed based on the characterization of the data from six sampling sites. The microbial diversity of different points was analysed, using PCR amplification technology and T-RFLP technology. Generally, the physical and chemical properties of each sampling point in the interstitial water in the reservoir showed obvious difference. Different forms of nitrogen concentrations and organic carbon concentrations in the reservoir were lower than those of the external diversion and the tributary. The concentrations of the enviromental factors were in the orders of wet season>dry season>normal season. The result showed that bacterial community structures varied considerably between the upstream and the downstream. The source water behaved the higher level of bacterial community diversity and richer degree in dry season than that in normal season and wet season. The dominant bacteria were Family1_uncultured, Bacteroidetes_uncultured, Sphingobacteriales_uncultured, Anaerolineaceae in normal season, Peptostreptococcaceae_incertae_sedis, Anaerolineaceae, SC-I-84, Xanthomonadales, and Clostridium in wet season, Rhodocyclaceae, Fusobacteriales, BSV26_norank, Comamonadaceae, Anaerolineaceae in dry season. Bacterial diversity was rich in the sediments in the reservoir. Correlation analysis showed that the microbial community structure was different at different sites, effected by environmental factors, among which the effects of different forms of nitrogen concentration were most obvious.

Key words: reservoir, sediments, microbial diversity, environmental factors, correlation analysis

摘要：

为评价广东某水库底泥中微生物多样性及其与环境因子的相关性, 为该区域微生物的生态功能研究提供理论基础, 选择水库库中、外部引水入口及主要支流入口共6个采样点, 进行不同季节的水质特征分析, 结合PCR扩增技术和T-RFLP技术, 对不同时期各点水体中微生物进行多样性分析, 并分析微生物群落结构多样性与水库环境条件的关系。从总体上看, 水库底泥中各采样点间隙水的理化性质具有较高的时空差异性, 库中不同形式氮的浓度和有机碳浓度低于外部供水入口及支流, 各指标浓度大体上呈现丰水期>枯水期>平水期的现象。枯水期水库底泥的微生物比平水期和丰水期丰富, 不同时期不同采样点的细菌多样性分布也呈现不同规律。平水期的优势菌属主要有Family1_uncul- tured, Bacteroidetes_uncultured, Sphingobacteriales_uncultured和Anaerolineaceae, 丰水期的优势菌属主要有Peptostre- ptococcaceae_incertae_sedis, Anaerolineaceae, SCI-84, Xanthomonadales和Clostridium, 枯水期的优势菌属主要有Rhodocyclaceae, Fusobacteriales, BSV26_norank, Comamonadaceae和Anaerolineaceae。水库底泥中细菌具有丰富的多样性, 水体微生物群落结构在不同点位具有一定的差异性, 受环境因子的综合影响, 不同形式氮浓度的影响尤为明显。

关键词: 水库, 底泥, 微生物多样性, 环境因子, 相关性分析