Abstract: Metallothionein-3(MT-3), previously named as growth inhibitory factor, is the unique member of the metallothionein family that demonstrates bioactivity in vitro. To test if MT-3 dimerizes or polymerizes itself in vivo, the yeast two-hybrid system was established. In yeast two-hybrid system, MT-3 interacts with MT-3. A further two-hybrid test also indicated that MT-3 weakly interacted with its isoform MT-1. These primary results suggest that MT-3 probably trend to homodimerize or heterodimerize in vivo.

Key words: metallothionein-3, yeast two-hybrid system, interaction, dimerization

摘要： 金属硫蛋白-3(MT-3)，又称神经生长抑制因子，为金属硫蛋白家族中惟一具有生物活性的成员。为了验证其在细胞内是否形成二聚或多聚体，构建酵母双杂交系统用于检测。结果表明：MT-3与MT-3之间在酵母细胞内能发生相互作用，进一步用酵母双杂交实验还表明MT-3与MT-1间也存在弱相互作用。由此提示，在机体细胞中，MT-3可以以同源或异源二聚体的形式存在。

关键词: 金属硫蛋白-3, 酵母双杂交系统, 相互作用, 二聚体