Abstract: Via suspension culture and 0.5 μmol/L RA induction, we established an in vitro system to differentiate ES cells directly into neuron-like cells. In this system, neuron-like cells first appeared within 48h after the embryoid bodies were plated, and were most abundant 4-6 days after plating, while decreased 8-10 days later. Neuron-like cells differentiated from ES cells had typical morphologic properties, and specifically reacted with NF antibodies.

Key words: ES cells, in vitro differentiation, neuron

摘要： 通过悬浮培养和0.5μmol/L RA诱导，建立了定向诱导ES细胞向神经元样细胞分化的体系。在该体系中，神经元样细胞于类胚贴壁后48h之内出现，4～6d时达到最多，8～10d后减少。由ES细胞分化而来的神经元样细胞具有典型的形态特征，并且可以与NF抗体发生特异反应。

关键词: ES细胞, 体外分化, 神经元