Acta Scientiarum Naturalium Universitatis Pekinensis ›› 2016, Vol. 52 ›› Issue (5): 785-792.DOI: 10.13209/j.0479-8023.2016.036

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Determination of Seven Organic Acids in Juice by Retention Model in Ion Chromatography

LÜ Hongfeng, CUI Meilei   

  1. College of Science, China Agricultural University, Beijing 100083
  • Received:2015-04-14 Revised:2015-07-30 Online:2016-09-20 Published:2016-09-20
  • Contact: LÜ Hongfeng, E-mail: hongfen(at)


吕洪凤, 崔梅蕾   

  1. 中国农业大学理学院, 北京 100083
  • 通讯作者: 吕洪凤, E-mail: hongfen(at)
  • 基金资助:
    国家自然科学基金(11474355, 60977057)和中国农业大学URP 项目资助


Optimal chromatography condition can be obtained via time-consuming experiments. To simplify it, an improved retention model assisting experiments optimum are developed and the separating conditions of seven organic acids in juice are established. The retention behaviors of organic acids on AS11-HC analytical columns are analyzed. It is found that the gradient model performs well for linear gradient elution while it should be assisted with a trial-and-error optimization for nonlinear gradient elution.

Key words: words retention model, organic acids, juice, ion chromatography


离子色谱是检测果汁内有机酸的重要辨别法, 但需要反复试验以确定最优色谱条件。为缩短时间、降低成本, 首次将离子色谱保留模型应用于果汁有机酸的分析中, 在理论和实验相结合的情况下提出优化模型, 随后确立该模型分析果汁内7 种有机酸的最优色谱条件。在该条件下, 对苹果汁中常见的7 种有机酸进行检测, 对 3 种不同的苹果汁进行分析, 验证了该方法的精准性和适用性。研究结果可为果汁内有机酸的检测提供一定的理论支撑。

关键词: 保留模型, 有机酸, 果汁, 离子色谱

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