S100A4和S100A10在小鼠毛囊发育中的表达定位

doi:10.13209/j.0479-8023.2024.106

北京大学学报自然科学版 ›› 2025, Vol. 61 ›› Issue (3): 456-464.DOI: 10.13209/j.0479-8023.2024.106

S100A4和S100A10在小鼠毛囊发育中的表达定位

白勇将¹, 栗雯华¹, 杨炜峰², 崔云丽¹, 叶富豪¹, 侯增耀¹, 庞全海^1,†

1. 山西农业大学动物医学学院, 晋中 030801 2. 北京维通达生物技术有限公司, 北京 102200

收稿日期:2024-03-01 修回日期:2024-05-21 出版日期:2025-05-20 发布日期:2025-05-20
通讯作者: 庞全海, E-mail: pangquanhai(at)126.com
基金资助:
国家自然科学基金(31872532, 31272628)资助

Expression and Localization of S100A4 and S100A10 in Mouse Hair Follicle Development

BAI Yongjiang¹, LI Wenhua¹, YANG Weifeng², CUI Yunli¹, YE Fuhao¹, HOU Zengyao¹, PANG Quanhai^1,†

1. College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801 2. Vitalstar Bitechnology Co., Ltd, Beijing 102200

Received:2024-03-01 Revised:2024-05-21 Online:2025-05-20 Published:2025-05-20
Contact: PANG Quanhai, E-mail: pangquanhai(at)126.com

摘要/Abstract

摘要：

为了研究S100A4和S100A10在C57BL/6N小鼠皮肤毛囊发育不同时间点的表达与定位, 选取E13.5, E15.5, E18.5孕鼠胚胎和新生鼠P2, P4, P8小鼠, 每个发育时间点各取3只小鼠, 取背部皮肤组织, 通过分子生物学及组织学技术, 检测不同时期小鼠背部皮肤中S100A4和S100A10 mRNA及蛋白的表达与定位。结果表明, 在毛囊发育时期, S100A4和S100A10的mRNA和蛋白表达均先升高后降低, 出生2天时的mRNA和蛋白表达最高。免疫组织化学定位显示, S100A4蛋白和S100A10蛋白在小鼠毛囊发育中持续表达, 但是定位区域有所不同, 提示S100A4和S100A10可能在小鼠毛囊发育过程中具有重要的作用。综上所述, S100A4和S100A10在小鼠毛囊发育不同时间点的背部皮肤中表达不同, S100A4和S100A10参与小鼠毛囊发育过程。

关键词: S100A4, S100A10, 小鼠, 毛囊发育

Abstract:

In order to study the expression and localization of S100A4 and S100A10 at different time points of skin hair follicle development in C57BL/6N mice, E13.5, E15.5, E18.5 pregnant mouse embryos and P2, P4, P8 newborn mice were selected. Three mice were taken at each time-point. The expression and localization of S100A4 and S100A10 mRNA and proteins in the back skin of mice were detected by molecular biology and histological techniques. The results showed that the mRNA and protein expressions of S100A4 and S100A10 firstly increased and then decreased during the development of hair follicles, and the mRNA and protein expressions were the highest at 2 days after birth. Immunohistochemical localization showed that S100A4 protein and S100A10 protein were continuously expressed in mouse hair follicle development, but their localization regions were different. It is suggested that S100A4 and S100A10 may play an important role in the development of mouse hair follicles. In conclusion, the expression of S100A4 and S100A10 is different in the back skin of mice at different time points of hair follicle development, and S100A4 and S100A10 participate in the development process of mouse hair follicles.

Key words: S100A4, S100A10, mice, hair follicle development

白勇将, 栗雯华, 杨炜峰, 崔云丽, 叶富豪, 侯增耀, 庞全海. S100A4和S100A10在小鼠毛囊发育中的表达定位[J]. 北京大学学报（自然科学版）, 2025, 61(3): 456-464.

BAI Yongjiang, LI Wenhua, YANG Weifeng, CUI Yunli, YE Fuhao, HOU Zengyao, PANG Quanhai. Expression and Localization of S100A4 and S100A10 in Mouse Hair Follicle Development[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2025, 61(3): 456-464.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://xbna.pku.edu.cn/CN/10.13209/j.0479-8023.2024.106

https://xbna.pku.edu.cn/CN/Y2025/V61/I3/456

[1]	滕春波, 宋晓明, 马红, 杨增明. 促性腺激素处理对性未成熟小鼠子宫中STAT3表达和激活的影响[J]. 北京大学学报（自然科学版）, 2004, 40(4): 565-572.
[2]	汤富酬,薛友纺,李美琴,尚克刚. DNA指纹图在小鼠ES细胞嵌合体鉴定中的应用[J]. 北京大学学报（自然科学版）, 2000, 36(6): 789-794.
[3]	童英, 邹冀中, 尚克刚. 用大鼠心肌条件培养基建立来源于C57BL/6J小鼠的ES细胞系[J]. 北京大学学报（自然科学版）, 2000, 36(4): 472-476.
[4]	韩嵘, 柴桂萱, 尚克刚. 大鼠心肌条件培养基对形成小鼠ES细胞集落的影响[J]. 北京大学学报（自然科学版）, 1997, 33(2): 185-188.
[5]	周未艾, 高天礼 . 小鼠心脏缺灌和再灌早期乳酸脱氢酶释放规律[J]. 北京大学学报（自然科学版）, 1996, 32(2): 239-247.
[6]	胡新立, 尚克刚 . 6个小鼠ES细胞系的建立和鉴定[J]. 北京大学学报（自然科学版）, 1996, 32(2): 248-253.

S100A4和S100A10在小鼠毛囊发育中的表达定位

Expression and Localization of S100A4 and S100A10 in Mouse Hair Follicle Development

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

留言