The study aimed to examine the effects of patient expectation on patient trust in physician-patient interaction scenarios from public and patient perspectives. Study 1 was based on the public perspective. Four groups of 12 physician-patient interaction scenarios were constructed to examine the effects of patients’ expectations on their trust. The study sample included 114 people (M=24 years, SD=7.25; 71 females). Study 2 was based on the patient perspective and included 50 patients (M=23.96 years, SD=6.51; 26 females) who needed wisdom teeth extraction. Two sets of patient-physician interaction scenarios were constructed, and the effect of whether expectations were met in terms of patient trust was explored. The results showed that 1) The main effect of conforming to expectations regarding patient trust was significant, but the main effect of high or low levels of patients’ expectations on patient trust was not significant. 2) No interaction between high/low expectations and whether to conform to expectations was found. 3) The effect of whether expectations regarding patients’ trust were met was significant in the group that required wisdom teeth extraction. Therefore, conforming to patients’ expectations plays an important role in establishing patient trust, and patients whose physicians conform to their expectations have greater trust in their physicians. In the future, healthcare professionals can interact and communicate more with patients, pay attention to their real needs and expectations, actively allocate healthcare resources to meet these needs, enhance patient satisfaction and trust, and establish a harmonious healthcare relationship.