水稻幼苗, 褐藻胶寡糖, 抗寒性能, 蛋白质组学, 代谢组学

Nipponbare rice was selected as the research object, and the whole process of rice seedlings from normal growth to low temperature to recovery was simulated. In combination with the determination of physiological and biochemical indicators of rice seedlings, it was found that the application of alginic oligosaccharides not only improved the ability of rice seedlings to resist low temperature stress, but also improved root vitality and the activities of SOD, POD, CAT. At the same time, when rice seedlings are subjected to low temperature stress, alginate oligosaccharides could promote the production of soluble sugars and effectively inhibit the increase of malon-dialdehyde content in rice seedlings. Label-free quantification was used to identify and analyze differential proteins and metabolites and study their metabolic pathways. After GO annotation of differentially expressed proteins, the experimental group enriched more entries. The first 10 key pathways were obtained by KEGG enrichment. Metabolomic analysis of rice seedlings by UPLC-Q-TOF-MS showed that the main small molecule differential metabolites were amines, followed by carboxylic acids and alkaloids. Through multiomics analysis, the mechanism of alginate oligosaccharides in improving the cold resistance of rice seedlings was elucidated, which provided a scientific basis for understanding the defense mechanism of cold resistance of rice seedlings as well as the development and utilization of alginate oligosaccharides.

rice seedlings, alginate oligosaccharides, cold resistance performance, proteomics, metabolomics