Through the development of plant culture and imaging system, a low-cost plant living fluorescence imaging system was developed. The system adopts a double-layer box structure design, which is divided into two parts: the constant culture system and the fluorescence recording system. It can provide the necessary life support function for plant growth, and can also obtain the key images in the process of plant growth in real time, including the time-space mode of monitoring the fluorescence signal in the development of the transgenic plant. The EGFP transgenic tobacco and Arabidopsis were successfully cultured and observed in real time using this system. The low cost greatly expanded the application of the system in experimental teaching, and also laid a foundation for the further study of the observation and detection of follow-up life activities.

instrument development, in-vivo fluorescence imaging system for living plants, plant cultivation real-time microscope recorder, plant incubator