In order to explore the changing trend and response mechanism of karst carbon sinks under the background of climate change and rocky desertification control engineering, this paper estimates the temporal and spatial pattern of karst carbon sinks in Guangxi from 2005 to 2020 based on Guangxi lithologic geological data and meteorological data, and further The effects of climate change and rocky desertification control on karst carbon sinks are analyzed. The results show that from 2005 to 2020, the temporal and spatial differences of karst carbon sinks in Guangxi are significant, showing a trend of stable fluctuation and rising with an average annual increase of 8.1%. Precipitation has the most significant impact on the changes of karst carbon sinks while temperature and evapotranspiration have little effect. The responses to the control measures are different. As the most common control measures for rocky desertification, closing the mountain for management and protection has the most significant role in promoting carbon sequestration and increasing sinking with an average annual growth contribution rate of about 28%.