北京大学学报自然科学版 ›› 2020, Vol. 56 ›› Issue (5): 966-970.DOI: 10.13209/j.0479-8023.2020.059

上一篇    下一篇

BDE-209对人正常肝L-02细胞生长和凋亡的影响

朱婷婷1, 陆永奋2, 齐秀娟1, 黄毅1, 孟海涛3, 尹东高1, 孙滔滔1, 邓臣1,†, 吴德生4,†   

  1. 1. 国家环境保护饮用水水源地管理技术重点实验室, 深圳市水环境中新型污染物检测与控制重点实验室, 广东省低能耗污水处理工程技术研究中心, 深圳市环境科学研究院, 深圳 518001 2. 深圳大学化学与环境工程学院, 深圳 518060 3. 淄博职业学院化学工程系, 淄博 255314 4. 深圳市疾病预防控制中心毒理研究所现代毒理学重点实验室, 深圳 518055

  • 收稿日期:2019-08-13 修回日期:2020-05-18 出版日期:2020-09-20 发布日期:2020-09-20
  • 通讯作者: 邓臣, E-mail: 185390228(at)qq.com; 吴德生, E-mail: dswucn(at)126.com
  • 基金资助:
    深圳市知识创新计划基础研究项目(JCYJ20160531114757157)和深圳市环境科研课题(SZCG2019194864)资助

Effects of Decabrominated Diphenylether-209 on Cell Proliferation and Cell Apoptosis in Human Liver L-02 Cells

ZHU Tingting1, LU Yongfen2, QI Xiujuan1, HUANG Yi1, MENG Haitao3, YIN Donggao1, SUN Taotao1, DENG Chen1,†, WU Desheng4,†   

  1. 1. State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology, Shenzhen Key Laboratory of Emerging Contaminants Detection and Control in Water Environment, Guangdong Engineering Research Center of Low Energy Sewage Treatment, Shenzhen Academy of Environmental Sciences, Shenzhen 518001

    2. College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060

    3. Zibo Vocational Institute, Zibo 255314

    4. Key Laboratory of Modern Toxicology of Shenzhen, Institute of Toxicology, Shenzhen Centre for Disease Control and Prevention, Shenzhen 518055

  • Received:2019-08-13 Revised:2020-05-18 Online:2020-09-20 Published:2020-09-20
  • Contact: DENG Chen, E-mail: 185390228(at)qq.com; WU Desheng, E-mail: dswucn(at)126.com

PDF

摘要：

采用Cell Counting Kit-8 (CCK-8)和Hoechst 33342/PI等, 分析十溴联苯醚(BDE-209)对人正常肝L-02细胞增殖和凋亡的影响。结果显示, 在不同浓度水平(0~70 μg/mL)的BDE-209中暴露24小时后, 细胞增殖抑制率随着BDE-209浓度的升高而升高。经SPSS-Probit-Logit法计算, 得出 BDE-209对人正常肝L-02细胞24小时的IC50为127.08 ±24.93 μg/mL。在不同浓度水平(0~15 μg/mL)的BDE-209中暴露24小时后, 与对照组相比, 各剂量组细胞未出现明显的细胞形态变化, 仅在15 μg/mL剂量组观察到细胞减少的现象。凋亡染色后, 荧光显微镜检发现, 随着BDE-209浓度升高, 亮蓝、淡粉染色细胞数量增大, 人正常肝L-02细胞的凋亡率增高。与对照组相比, 各剂量组差异均具有统计学意义。分析结果表明, BDE-209可引起人正常肝L-02细胞增殖受到抑制, 并诱导细胞凋亡率增加。

关键词: BDE-209, 人肝L-02细胞, 细胞增殖, 细胞凋亡

Abstract:

The effects of BDE-209 (decabrominated diphenylether-209) on the proliferation and apoptosis of human normal liver L-02 cells were analyzed by CCK-8 and Hoechst 33342/PI methods. The results showed that the inhibition rate of cell proliferation increased with the increase of BDE-209 concentration after 24 hours exposure at different concentration levels (0–70 μg/mL). The IC50 of BDE-209 on human normal L-02 cells for 24 hours was 127.08±24.93 μg/mL, which was analyzed by SPSS-Probit-Logit method. After 24 hours exposure to BDE-209 at different concentration levels (0–15 μg/mL), there was no significant change in cell morphology in each dose group compared with the control group, and only cell reduction was observed in the 15 μg/mL dose group. After apoptotic staining, fluorescence microscopy showed that the number of cells stained with brilliant blue and light powder increased with the increase of BDE-209 concentration, and the apoptosis rate of human normal liver L-02 cells increased with the increase of BDE-209 concentration. Compared with the control group, the difference of each dose group was statistically significant. The results showed that BDE-209 could inhibit the proliferation of human normal liver L-02 cells and increase the apoptotic rate.

Key words: decabrominated diphenylether-209, human liver L-02 cell, cell proliferation, cell apoptosis

版权所有 © 《北京大学学报（自然科学版）》编辑部
技术支持：北京玛格泰克科技发展有限公司