基于Riboswitch的细胞与门构建

北京大学学报（自然科学版）

基于Riboswitch的细胞与门构建

陈梅,张路,许进

北京大学信息科学技术学院软件研究所, 高可信软件技术教育部重点实验室, 北京 100871;

收稿日期:2013-04-25 出版日期:2014-05-20 发布日期:2014-05-20

Construction of a Cellular AND Gate Using Riboswitch

CHEN Mei, ZHANG Lu, XU Jin

Software Institute, School of Electronics Engineering and Computer Science, Peking University, Key Laboratory for High Confidence Software Technologies of Ministry of Education, Beijing 100871;

Received:2013-04-25 Online:2014-05-20 Published:2014-05-20

摘要/Abstract

摘要： 利用Riboswitch调控原理, 在大肠杆菌BL21(DE3)菌株中构建一个细胞与门。该与门以实验室常见的阿拉伯糖和异丙基-β-D-硫代吡喃半乳糖苷(IPTG)为输入信号, 黄色荧光蛋白YFP为输出信号, 流式细胞仪检测结果显示该细胞与门成功地实现与逻辑运算。该与门利用大肠杆菌BL21(DE3)菌株的特性, 减少了基因线路的负载。此外, 该与门还具有可复用的特点。

关键词: 生物分子计算, 逻辑门, 大肠杆菌BL21(DE3)菌株, 核糖开关

Abstract: A cellular AND logic gate was constructed in Escherichia coli BL21 (DE3) using Riboswitch. In this AND gate, arabinose as well as isopropyl-β-d-thiogalactoside (IPTG) were used as inputs, and yellow fluorescent protein (YFP) was used as output. Flow cytometry results show that the AND gate successfully realizes the AND logic. This AND gate uses the characteristic of BL21 (DE3) to reduce the load of genetic circuits. Besides, this AND gate can be reused.

Key words: biology molecule computing, logic gates, E.coli BL21 (DE3), Riboswitch

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