Pseudomonas sp. QG6降解喹啉过程中除磷特性及影响因素研究

北京大学学报（自然科学版）

Pseudomonas sp. QG6降解喹啉过程中除磷特性及影响因素研究

段明君¹,倪晋仁^1,2

1. 北京大学深圳研究生院环境与能源学院, 深圳 518055; 2. 北京大学环境工程系, 水沙科学教育部重点实验室, 北京 100871;

收稿日期:2013-03-10 出版日期:2014-03-20 发布日期:2014-03-20

Phosphate Removal by a Quinoline-Biodegrading Bacterium: Characteristics and Influence Factors

DUAN Mingjun¹, NI Jinren^1,2

1. School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055; 2. Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences MOE,Beijing 100871;

Received:2013-03-10 Online:2014-03-20 Published:2014-03-20

摘要/Abstract

摘要： 从工业废水处理系统中分离出一株以喹啉为唯一碳源和氮源的假单胞菌QG6 (Pseudomonas sp. QG6), 用于喹啉降解同步除磷, 并采用正交实验设计优化出最佳条件。菌株QG6具有较好的喹啉降解能力, 12小时内能将96~144 mg/L的喹啉完全降解。菌株QG6在好氧条件下具有除磷能力, 不存在喹啉的条件下, 以有机碳为碳源、无机氮为氮源、初始磷酸盐浓度为8.69~19.41 mg/L时, 20小时内能去除磷酸盐86%以上。初始喹啉浓度为144 mg/L (其自身的碳氮比约7:1)、磷酸盐浓度为10 mg/L时, 若不外加有机碳源, 喹啉在12小时内被降解完全, 同一时段内除磷率仅为33%。外加有机碳源至碳氮比20:1且其他条件都相同时, 喹啉降解效果不受影响, 且同步除磷率提高到 86%。正交实验表明, 外加碳源条件下喹啉降解的最佳条件按影响大小排列为: 初始喹啉浓度200 mg/L, 温度25°C, pH 8, 摇床转速120 rpm; 除磷最佳条件为: 摇床转速100 rpm, 温度为25°C, 初始喹啉浓度150 mg/L, pH 9。

关键词: Pseudomonassp.QG6, 喹啉降解, 除磷, 正交试验

Abstract: Pseudomonas sp. QG6, which could utilize quinoline as sole carbon and nitrogen source, was isolated from industrial wastewater. The best conditions for simultaneously biodegrading quinoline and removing phosphorus were optimized through orthogonal experiment. Strain QG6 displayed excellent capability of biodegrading quinoline, i.e., the biodegradation process could be completed in 12 hours with initial quinoline concentration of 96?144 mg/L. With organic carbon as carbon source, inorganic nitrogen as nitrogen source and no quinoline added, the removal efficiency of 8.69?19.41 mg/L of phosphate could reach 86% in 20 hours, suggesting strain QG6 displayed perfect phosphate removal in aerobic condition. With no external organic carbon source added, the simultaneous phosphate removal efficiency of 10 mg/L was only 33% though the degradation of quinoline (C/N=7:1) with initial concentration of 144 mg/L showed little decrease. Adding external organic carbon source to initial C/N of 20:1 significantly improved phosphorus removal when simultaneously degrading quinoline, i.e., the removal efficiency of phosphate significantly increased up to 86%. Results of orthogonal experiment display that with degree of external carbon source, the optimum pH, temperature (T), rotation speed of shaking table (S) and initial concentration of quinoline (C0) in order of importance are C0=200 mg/L, T=25°C, pH=8 and S=120 rpm for quinoline degradation; and S=100 rpm, T=25°C, C0=150 mg/L and pH=9 for phosphate removal, respectively.

Key words: strain QG6, quinoline biodegradation, phosphate removal, orthogonal experiment

中图分类号:

X172

段明君,倪晋仁. Pseudomonas sp. QG6降解喹啉过程中除磷特性及影响因素研究[J]. 北京大学学报（自然科学版）.

DUAN Mingjun,NI Jinren. Phosphate Removal by a Quinoline-Biodegrading Bacterium: Characteristics and Influence Factors[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://xbna.pku.edu.cn/CN/

https://xbna.pku.edu.cn/CN/Y2014/V50/I2/361

[1]	陶虎春, 佟浩, 王健, 黄奕龙, 张丽娟, 杨凯, 丁凌云, 乔雪姣, 豆敏, 许楠. 碳氮比对A/O-MBR工艺中污水脱氮除磷的影响研究[J]. 北京大学学报自然科学版, 2022, 58(4): 680-686.
[2]	邓若男,陈倩,倪晋仁. 高盐废水处理的耐盐菌株及其高效除磷特性研究[J]. 北京大学学报（自然科学版）, 2013, 49(5): 880-884.
[3]	郎宇琪,冯波,秦春梅,王健,其鲁. 微波辅助碱氧化锡林浩特褐煤正交实验研究[J]. 北京大学学报（自然科学版）, 2012, 48(1): 169-172.
[4]	彭赵旭, 彭永臻, 苏欣颖, 桂丽娟,王淑莹,刘洋. 黏性膨胀下活性污泥的脱氮除磷特点研究[J]. 北京大学学报（自然科学版）, 2010, 46(3): 417-421.
[5]	季兵,陈季华. 人工湿地系统处理上海崇明高浊度富营养化水体的研究[J]. 北京大学学报（自然科学版）, 2010, 46(3): 407-412.
[6]	刘宝河,张林生,孟冠华,郑俊. TBX 多孔陶粒滤料制备及废水吸附除磷试验研究[J]. 北京大学学报（自然科学版）, 2010, 46(3): 389-394.
[7]	邱向阳,陆文静,王洪涛,黄鼎曦,黄得扬,高海军. 蔬菜—花卉秸秆混合堆肥性状表征及纤维素分解菌群选育研究[J]. 北京大学学报（自然科学版）, 2003, 39(2): 254-261.