高盐废水处理的耐盐菌株及其高效除磷特性研究

北京大学学报（自然科学版）

高盐废水处理的耐盐菌株及其高效除磷特性研究

邓若男,陈倩,倪晋仁

北京大学环境工程系, 水沙科学教育部重点实验室, 北京 100871;

收稿日期:2012-09-21 出版日期:2013-09-20 发布日期:2013-09-20

Efficient Phosphorus Removal from Wastewater by a Newly Isolated Bacterium under High Salinity Condition

DENG Ruonan, CHEN Qian, NI Jinren

Key Laboratory of Water and Sediment Sciences MOE, Department of Environmental Engineering, Peking University, Beijing 100871;

Received:2012-09-21 Online:2013-09-20 Published:2013-09-20

摘要/Abstract

摘要： 筛选出一株能够高效除磷的耐盐菌株HG-1。通过个体形态、菌落特征的观察和16S rRNA基因的序列分析, 初步鉴定为盐弧菌属(Salinivibrio sp.)。对菌株HG-1的耐盐性能及其在不同盐度下对磷酸盐的去除效果进行考察, 结果表明菌株对盐度的耐受范围为1%~13%, 最适盐度为3%。进一步的单因素和正交实验表明, 4个环境因素对菌株HG-1磷酸盐去除率影响的强弱为: pH>碳氮比>温度>接种量, 最优的除磷条件为pH 6.5~7.0, 温度30℃, 接种量10%, 碳氮比9, 在该条件下菌株在24小时内对磷酸盐的去除率可达100%。将该菌株应用于高盐废水的处理, 可实现磷酸盐的有效去除, 具有良好的实际应用价值, 为高盐条件下生物除磷难题的解决提供了一条新的途径。

关键词: 除磷, 高盐废水, 耐盐菌

Abstract: A bacterium capable of phosphorus removal was isolated. Through morphology observation and 16S rRNA gene sequence analysis, the isolate was identified as Salinivibrio sp. (named HG-1). Salinity tolerance and phosphorus removal efficiency under different salinity conditions of the strain were further investigated. The results showed that HG-1 grew well with the salt content varying from 1% to 13% and achieved the highest phosphorus removal efficiency under salt content of 3%. Furthermore, the single-factor and orthogonal experiment results indicated that the optimal phosphorus removal performance was obtained under the conditions with an initial pH of 6.5?7.0, C/N ratio of 9, temperature of 30°C and inoculation ratio of 10%. Under such a condition, the phosphorus removal efficiency could reach 100% in 24 hours. The strain HG-1 can independently complete phosphorus removal process, and thus could provide a novel and promising alternative for biological phosphorus removal under high salinity conditions.

Key words: phosphorus removal, high salinity wastewater, salt tolerant strain

中图分类号:

X172

邓若男,陈倩,倪晋仁. 高盐废水处理的耐盐菌株及其高效除磷特性研究[J]. 北京大学学报（自然科学版）.

DENG Ruonan,CHEN Qian,NI Jinren. Efficient Phosphorus Removal from Wastewater by a Newly Isolated Bacterium under High Salinity Condition[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

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链接本文: https://xbna.pku.edu.cn/CN/

https://xbna.pku.edu.cn/CN/Y2013/V49/I5/880

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