北京大学学报（自然科学版）

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布吉河枯水期总细菌和反硝化功能基因定量研究

孙海美1,2,孙卫玲2,邵军1,2   

  1. 1. 北京大学深圳研究生院环境与能源学院, 城市人居环境科学与技术重点实验室, 深圳518055; 2. 北京大学环境科学与工程学院, 水沙科学教育部重点实验室, 北京100871;
  • 收稿日期:2011-07-28 出版日期:2012-07-20 发布日期:2012-07-20

Quantifying Total Bacteria and Functional Genes of Denitrifying Bacteria in Buji River in Dry Season

SUN Haimei1,2, SUN Weiling2, SHAO Jun1,2   

  1. 1. Key Laboratory for Urban Habitat Environmental Science and Technology, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055; 2. College of Environmental Sciences and Engineering, Peking University, Key Laboratory of Water and Sediment Sciences MOE, Beijing 100871;
  • Received:2011-07-28 Online:2012-07-20 Published:2012-07-20

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摘要： 为了研究污染河流中微生物群落结构的存在状况及与水环境因子的关系, 以深圳市深圳河的一级支流布吉河为研究对象, 采用DGGE和Q-PCR技术分析了布吉河不同断面水样和沉积物中总细菌数量和群落结构以及反硝化基因(nosZ和narG基因)数量变化。结果表明, 水样中总细菌数量的变化范围为5.77×108~ 7.13×1011 copies/L, nosZ和narG基因数量的变化范围分别为2.99×106~9.39×108 copies/L和1.45×107~9.11×109 copies/L。沉积物中总细菌数量的变化范围为1.14×109~1.61×1011 copies/g, nosZ和narG基因数量的变化范围分别为1.52×106~3.80×107 copies/g和2.38×107~2.93×108 copies/g。由于生境的差异, 沉积物中nosZ和narG基因丰度高于水样。冗余度分析表明, 影响微生物数量和群落结构的水环境因子不同, Fe是影响布吉河水样中细菌数量的主要水环境因子, 而COD、亚硝氮和硝氮是影响布吉河微生物群落结构的主要水环境因子。水样和沉积物中微生物生态功能都很稳定, 但水样中总细菌多样性比沉积物中高。

关键词: 荧光定量PCR, DGGE, 总细菌, 反硝化细菌, 布吉河

Abstract: To explore the bacteria community structure and it’s relation with water quality in polluted rivers, water and sediment samples were collected from Buji River (Shenzhen) in dry season. Quantification of total bacteria and denitrifying bacteria based on nosZ and narG gene were performed by real-time PCR, and the microbial community structures were studied by PCR-DGGE. The results show that the total bacteria 16S rRNA fragment changed from 5.77×108 to 7.13×1011 copies/L in water samples and from 1.14×109 to 1.61×1011 copies/g in sediments. The copy numbers of nosZ gene varied from 2.99×106 to 9.39×108 copies/L in water samples, and from 1.52×106 to 3.80×107 copies/g in sediments. The copy numbers of narG gene were in the range from 1.45×107 to 9.11×109 copies/L in water samples and 2.38×107 to 2.93×108 copies/g in sediments. The gene abundance of nosZ and narG genes in sediment was higher than that in water. Redundancy discrimination analysis (RDA) shows that Fe was the main factor influencing the numbers of bacteria, while COD, nitrite, and nitrate were the main factors affecting the microbial community structures in Buji River. The diversity of bacteria in water samples was higher than that in sediments.

Key words: real-time PCR, DGGE, total bacteria, denitrifying bacteria, Buji River

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