关键词: 惊厥, 远期, N-甲基-D-天冬氨酸, 受体, mRNA

Abstract: To explore long-term changes of NMDAR subunit mRNA expression induced by early magnesium-free treatment in developing cortical neurons of rat. The seizure model was recurrent epileptiform discharges induced by transient magnesium-free media treatment in the primary cultured embryo cortical neurons of rat. According to different treatment to cortical neurons at cultured day 6, three groups were divided: regular DMEM medium (CONT1)、regular extracellular solution medium (CONT2) and magnesium-free extracellular solution medium (MGF). The neuronswere exposed to above three media for 3h in cultured day 6, and then cultured in regular DMEM medium again. The expression of NMDAR subunit NR1, NR2A, and NR2B mRNA was measured using fluorescent real-time quantitative RT-PCR at different cultured day (7、12、17). Our results in group MGF showed that NR1 mRNA expression decreased at d7 and increased at d12 (p<0.05), NR2A mRNA expression decreased at d12 and increased at d17 (p<0.05), and NR2B mRNA expression increased at d7 and d17 (p<0.05). There were not statistic significant differences of mRNA expression of NR1 at d17, NR2A at d7, and NR2B at d12 among three groups (p>0.05). There were significant alterations of proportion of NR1/NR2A, NR1/NR2B and NR1/NR2A/NR2B mRNA expression at d7, d12 and d17 in group MGF. The change of NMDAR subunit mRNA expression may be resulted by long-term changes of early magnesium-free treatment in developing cortical neurons of rat.

Key words: seizure, long-term, N-methyl-D-aspartate, receptors, mRNA