北京大学学报（自然科学版）

共刺激分子B7和CD40对日本血吸虫感染小鼠Th1/Th2免疫偏移的调控作用

夏超明1, 龚唯1, 骆伟1, 田芳1, 张学光2   

  1. 1苏州大学医学院寄生虫学教研室, 苏州, 215007; 2苏州大学医学院免疫学教研室, 苏州, 215007
  • 收稿日期:2003-11-12 出版日期:2004-07-20 发布日期:2004-07-20

Effects of B7 and CD40 Costimulatory Molecules on Th1/Th2 Immune Deviation of Mice Infected with Schistosoma japonicum

LIU Shuqun, SUN Zhirong   

  1. Institue of Bioinformatics, Department of Biological sciences and Biotechnology, Tsinghua University, Beijing, 100084
  • Received:2003-11-12 Online:2004-07-20 Published:2004-07-20

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摘要： 为观察干预B7/CD28和CD40/CD40L共刺激信号对Th1/Th2细胞因子表达水平和Th1/Th2免疫偏移的调控作用, 分别取小鼠感染日本血吸虫后6、8、10和12周的脾淋巴细胞经抗CD80（B7-1）mAb、抗CD86（B7-2）mAb、抗CD40mAb和抗CD40LmAb处理后培养72h, 用ELISA双抗体夹心法测定培养上清中IFN-γ和IL-4的表达水平的动态变化并分析干预2种不同的共刺激信号对Th1/Th2免疫偏移的影响。结果显示抗CD80mAb和抗CD86mAb均能显著抑制IL-4的表达水平，尤其是抗CD86mAb对IL-4抑制作用尤为明显。抗CD40mAb和抗CD40LmAb也能影响Th2细胞因子的表达。其中以阻断CD40分子的作用更为显著。结果提示B7/CD28和CD40/CD40L共刺激信号可以调节Th1/Th2细胞因子的表达水平和调控Th1/Th2免疫偏移。干预B7/CD28和CD40/CD40L介导的共刺激信号调控Th1/Th2免疫偏移有可能是一种控制血吸虫卵肉芽肿病变新型的免疫治疗途径。

关键词: 日本血吸虫, Th1/Th2细胞因子, 免疫偏移, B7, CD40

Abstract: To observe the effect of B7 and CD40 costimulatory molecules on Th1/Th2 cytokine expression levels and immune deviation in the mice infected with Schistosoma japonicum. Murine spleen lymphocyte cultures were made at 6, 8, 10 and 12 weeks after infection of the mice infected with Schistosoma japonicum. The cultures were incubated with anti-CD80mAb, anti-CD86mAb, anti-CD40mAb and anti-CD40LmAb for 72h. The kits of ELISA were used for the quantitative determination IFN-γ and IL-4 in the spleen lymphocyte culture medium. The results of analysis by the Th2 differentiation index that used to evaluate the immune balance of Th1/Th2 cells. The results showed that anti-CD80mAb and anti-CD86mAb can significantly inhibit the expression of IL-4 (p<0.01),and the effect of anti-CD86mAb is more significant than that of anti-CD80mAb. Anti-CD40mAb and ant-CD40LmAb can also significantly inhibit the expression of IL-4 (p<0.01), and the effect of anti-CD40mAb is more significant than that of anti-CD40LmAb. These findings suggest that the interference of costimulatory signals can modulate Th1/Th2 cytokine expression levels and immune deviation in the mice infected with Schistosoma japonicum. A novel potential approach is to control the egg granuloma formation of Schistosoma japonicumby B7 or CD40 as a mediator of Th1/Th2 immune deviation.

Key words: Schistosoma japonicum, Th1/Th2 type cytokine, immune deviation, B7, CD40

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