北京大学学报（自然科学版）

酵母双杂交系统测定抗雌激素物质

胡建英,谢国红   

  1. 北京大学环境学院资源与环境地理系，北京，100871
  • 收稿日期:2003-03-07 出版日期:2003-07-20 发布日期:2003-07-20

Determination of Chemical Compounds Using Antagonist Assay Based on Yeast Two-Hybrid System

HU Jianying, XIE Guohong   

  1. Dept. of Resource and Environmental Geosciences, College of Chemistry and Environmental Sciences, Peking University, Beijing, 100871
  • Received:2003-03-07 Online:2003-07-20 Published:2003-07-20

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摘要： 建立了用酵母双杂交系统筛选具有抗雌激素作用物质的生物测试法，并对6种工业用酚类物质(4-n-壬基酚，2-sec-丁基酚，2-羟基联苯，3-叔丁基酚，2-叔丁基酚和4-乙基2，6-二叔丁基酚)及氯化三苯基锡的抗雌激素作用进行了测试。结果表明：氯化三苯基锡显示出极强的抗雌激素作用，强度超过了抗乳癌剂三苯氧胺及其代谢产物4-羟基三苯氧胺。可以推测出有机锡造成软体动物的雄性化作用可能是由其抗雌激素作用引起的。6种酚类物质中，除4-乙基-2，6-二叔丁基酚外，其他5种显示出弱抗雌激素作用。值得注意的是：当4-壬基酚显示出雌激素促进作用时，4-n-壬基酚却显示出抗雌激素作用。

关键词: 酵母双杂交系统, 抗雌激素作用, 雌激素促进作用, 壬基酚, 有机锡

Abstract: The estrogenic antagonist activities of triphenyltin chloride and 6 phenolic com pounds (4-n-nonylphenol, 2-sec-butylphenol, 2-hydroxybiphenol, 3-tert-butylphenol, 2-tert-butylphenol and 4-methyl-2, 6-di-tert-butylphenol) used in the coating of concrete tanks and lining of still pipe in water supply system were detected using antagonist assay based on yeast two-hybrid system. It was found that triphenyltin chloride elicits strong antagonist activity of which is even higher than those of tamoxifen and its metabolized product, 4-hydroxytamoxifen. Of six phenolic compounds, five objective phenolic compounds elicited weaker estrogen antagonist activity except for 4-methyl-2, 6-di-tert-butylphenol. It should be noted that 4-n-nonylphenol elicited antagonist activity, while the 4-nonylphenol elicited agonist activity.

Key words: Yeast two-hybrid system, estrogenic antagonist, estrogenic agonist, nonylphenol, organotin

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