螅状独缩虫(Carchesium polypinum)类中间纤维的初步研究

北京大学学报（自然科学版）

螅状独缩虫(Carchesium polypinum)类中间纤维的初步研究

胡国群, 高崇明

北京大学生命科学学院，北京，100871

收稿日期:2002-04-08 出版日期:2003-03-20 发布日期:2003-03-20

The Evidence for the Existence of Intermediate-like Filaments in Carchesium polypinum

HU Guoqun, GAO Chongming

College of Life Sciences, Peking University, Beijing, 100871

Received:2002-04-08 Online:2003-03-20 Published:2003-03-20

摘要/Abstract

摘要： 利用间接免疫荧光和免疫印迹发现螅状独缩虫中存在两种分子质量为58ku和66ku的蛋白。这两种蛋白能分别与抗波形蛋白和抗核纤层蛋白B的抗体反应。分子质量66ku的蛋白位于大核边缘。另外，细胞经分级抽提后大核周围存在核纤层样结构。基于上述结果，可以认为螅状独缩虫中存在类中间纤维。

关键词: 螅状独缩虫, 类中间纤维, 核纤层, 波形蛋白, 核纤层蛋白B

Abstract: Using immuno-fluorescence labeling and immunoblot techniques, two intermediate filament-like proteins were identified in Carchesium polypinum. The molecular weights of these two proteins are 58ku and 66ku respectively. The 58ku protein reacts with anti-vimentin antibody and locates near the aboral wreath cilia. The other protein reacts with anti-lamin B antibody and is situated in the macronucleus. After the sequential fraction treatment, a nuclear lamina-like layer can be seen surrounding the macronucleus. Based on these results, it is concluded that intermediate-like filaments exist in Carchesium polypinum.

Key words: Carchesium polypinum, intermediate-like filaments, nuclear lamina, vimentin, lamin B

中图分类号:

Q242

胡国群, 高崇明. 螅状独缩虫(Carchesium polypinum)类中间纤维的初步研究[J]. 北京大学学报（自然科学版）.

HU Guoqun,GAO Chongming. The Evidence for the Existence of Intermediate-like Filaments in Carchesium polypinum[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

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https://xbna.pku.edu.cn/CN/Y2003/V39/I2/178