高浓度葡萄糖促进人二倍体成纤维细胞迅速衰老

北京大学学报（自然科学版）

高浓度葡萄糖促进人二倍体成纤维细胞迅速衰老

高云飞¹,周建军²,陈曦¹,赵允¹,苏小琴¹,刘诚³,翟中和¹

¹北京大学生命科学学院细胞生物学与遗传学系，北京，100871；²北京大学生命科学学院生物膜与膜生物工程

收稿日期:2001-07-06 出版日期:2002-07-20 发布日期:2002-07-20

Super-High-Concentration of Glucose Can Accelerate the Senescent Process of Human Diploid Fibroblast Cells

GAO Yunfei¹，ZHOU Jianjun²，CHEN Xi¹，ZHAO Yun¹，SU Xiaoqin¹，LIU Cheng³，ZHAI Zhonghe¹

¹Department of Cell Biology and Genetics, College of Life Sciences; ²National Key Lab of Biomembrane and Membrane Bioengineering, Peking University, Beijing, 100871; ³Chiron Corporation, CA 94608-2916 USA

Received:2001-07-06 Online:2002-07-20 Published:2002-07-20

摘要/Abstract

摘要： 利用光学显微镜观察和酸性β-半乳糖苷酶染色技术研究了高浓度葡萄糖对人二倍体成纤维细胞2BS细胞衰老进程的影响，并用流式细胞仪检测了此过程中活性氧和线粒体膜电位差的变化。结果表明：200 mmol/L的葡萄糖对2BS细胞有生长抑制作用，能引起活性氧含量的变化，导致线粒体膜电位差显著下降，并诱导了细胞的衰老。这为氧化损伤假说提供了新的证据，并为研究活性氧和复制衰老之间的关系提供了较好的体系。

关键词: 葡萄糖, 细胞衰老, 人二倍体成纤维细胞, 活性氧(ROS), 流式细胞术

Abstract: Light microtechnique and SA-β-galactosidase method was used to study the effects of super-high-concentration of glucose on the senescence of human diploid fibroblast 2BS cells, ROS and the membrane potential of mitochondria during this process were measured. Our results showed that 200 mmol/L of glucose inhibited the growth of 2BS cells, led to the changes of reactive oxygen species (ROS) and decrease of mitochondrial membrane potential,and caused senescence of 2BS cells rapidly. It supports the hypothesis of oxidative damage of senescence. Moreover it is a better system for the study of the effects of ROS during the process of replicative senescence.

Key words: glucose, cellular senescence, HDF, ROS, flow cytometry

中图分类号:

高云飞,周建军,陈曦,赵允,苏小琴,刘诚,翟中和. 高浓度葡萄糖促进人二倍体成纤维细胞迅速衰老[J]. 北京大学学报（自然科学版）.

GAO Yunfei,ZHOU Jianjun,CHEN Xi,ZHAO Yun,SU Xiaoqin,LIU Cheng,ZHAI Zhonghe. Super-High-Concentration of Glucose Can Accelerate the Senescent Process of Human Diploid Fibroblast Cells[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

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https://xbna.pku.edu.cn/CN/Y2002/V38/I4/572