北京大学学报（自然科学版）

雌三醇单克隆抗体的制备与表征

王永成1, 常文保1, 郭振泉2   

  1. 1北京大学化学与分子工程学院，生物有机与分子工程教育部重点实验室，北京，100871; 2北京大学生命科学中心，北京，100871
  • 收稿日期:2001-09-20 出版日期:2002-07-20 发布日期:2002-07-20

Production and Characterization of Anti-estriol Monoclonal Antibody

WANG Yongcheng1, CHANG Wenbao1, GUO Zhenquan2   

  1. 1The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871; 2Life Sciences Center, Peking University, Beijing, 100871
  • Received:2001-09-20 Online:2002-07-20 Published:2002-07-20

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摘要： 活化雌三醇(E3)C3处的酚—OH，与牛血清白蛋白(BSA)偶联，制备得E3的完全抗原。利用完全抗原免疫动物，采用单克隆抗体技术，经过细胞融合、克隆筛选、扩大培养、动物体内诱生等过程，最后获得了E3的单克隆抗体。对纯化的单克隆抗体的性质进行表征，结果表明：抗体的效价为6.0×10-10 mol/L，抗体属于IgG1型，分子量为168000 u，单克隆抗体与固定化抗原亲和常数为4.7×107 L/mol。

关键词: 细胞融合, 单克隆抗体, 雌三醇

Abstract: Estriol (E3) was bound to bovine serum albumin (BSA) to form a complete antigen (E3-BSA), after the hydroxy (-OH) at the position of C3 was activated. A BALB/c mouse was immunized with the mixture of E3-BSA and Freund adjuvant. Monoclonal antibody-producing hybridoma anti-E3 was prepared by fusion of SP2/0 murine myeloma cells with spleen cell isolated from the immunized mouse. A monoclonal antibody (McAb) specific for E3 was produced in vivo by the hybridoma anti-E3. Analysis revealed that anti-E3 McAb (E3-McAb) was of the IgG1 type,with the molecular weight 168000 u. The titer of E3-McAb was 6.0×10-10 mol/L, and the affinity constant of E3-McAb with coated complete antigen was 4.7×107 L/mol.

Key words: estriol, cell fusion, monoclonal antibody

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