抗丁草胺多克隆抗体的制备及应用

北京大学学报（自然科学版）

抗丁草胺多克隆抗体的制备及应用

郭永恩¹,陈家华¹,王能东¹,张秀¹,郭振泉²,金声¹

¹北京大学化学与分子工程学院，生物有机与分子工程教育部重点实验室，北京，100871；²北京大学生命科学学院，单克隆抗体实验室，北京，100871

收稿日期:2001-09-11 出版日期:2002-07-20 发布日期:2002-07-20

Preparation and Application of Polyclonal Antibody to Butachlor

GUO Yongen¹, CHEN Jiahua¹, WANG Nengdong¹, ZHANG Xiu¹, GUO Zhenquan², JIN Sheng¹

¹College of Chemistry & Molecular Engineering, Peking University, Beijing, 10871; ²College of Life Sciences, Peking University, Beijing, 100871

Received:2001-09-11 Online:2002-07-20 Published:2002-07-20

摘要/Abstract

摘要： 以牛血清白蛋白-丁草胺免疫抗原免疫家兔获得了抗丁草胺多克隆抗体，并建立了竞争酶联免疫吸附检测丁草胺(除草剂中的一种主要成分)分析方法。该方法的检测范围在1×10^-9～100×10^-9mg/mL之间，且和化学结构相似的甲草胺和乙草胺无交叉反应。该方法也可用于检测稻田水样中丁草胺的含量。

关键词: 多克隆抗体, 丁草胺, 酶联免疫吸附分析

Abstract: Polyclonal antibody to butachlor was prepared from rabbits immunized with butachlor-BSA conjugate, and a competitive ELISA was developed for the detection of the butachlor (a major component in a herbicide) within the range of 1×10^-9～100×10^-9mg/mL without cross reactions with the structurally similar chloroacetanilide herbicides, alachlor and acetochlor. This method could also be used to monitor the butachlor concentration of water samples from rice field.

Key words: polyclonal antibody, butachlor, ELISA

中图分类号:

R392.11

郭永恩,陈家华,王能东,张秀,郭振泉,金声. 抗丁草胺多克隆抗体的制备及应用[J]. 北京大学学报（自然科学版）.

GUO Yongen,CHEN Jiahua,WANG Nengdong,ZHANG Xiu,GUO Zhenquan,JIN Sheng. Preparation and Application of Polyclonal Antibody to Butachlor[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

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https://xbna.pku.edu.cn/CN/Y2002/V38/I4/447