关键词: 类核纤层蛋白, 草履虫

Abstract: Paramecium caudatumwas first processed by DGD embedment and embedment-free technique and general technique for EM morphology. A perinuclear structure consisted of interacted filaments (we called lamina-like structure) was observed. Then using Western Blot assay, we found a lamin-like component band of 68kD protein in the three-step-fractionated cells. To investigate the distribution of the lamin-like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti-lamin protein antibodies as the probes. The results revealed that the positive reactivity presented at different part of the cells. The perinuclear cross-actions were distinct, and cross-action with the oral apparatus and the cortex were also obtained.

Key words: lamin-like proteins, Paramecium