关键词: 同源性, 免疫荧光, 免疫印迹

Abstract: Anti-IF-protein antibodies were used as probes for immuno-fluorescence, and the reactions of them with different parts of the cell were detected, which suggested the possibility of the existence of the intermediate-like filaments in the cytoplasm. Those proteins homologous to the antibodies distributed regularly in the protoplasm. To characterize the corresponding proteins, SDS-PAGE and immunoblots were utilized. The 21, 23, 33 and 68kD proteins were distinguished among the diverse protein constituents of the cell. Some of these proteins also showed the cross-reactivities with anti-IF-proteins antibodies derived from higher organisms. These two evidences both contributed to the homology of some proteins in Paramecium to the intermediate filament proteins in higher organisms.

Key words: immunoblots, homology, immuno-fluorescence