北京大学学报(自然科学版)

DNA指纹图在小鼠ES细胞嵌合体鉴定中的应用

汤富酬,薛友纺,李美琴,尚克刚   

  1. 北京大学生命科学学院,遗传教研室,北京,100871
  • 收稿日期:1999-12-02 出版日期:2000-11-20 发布日期:2000-11-20

The Application of DNA Fingerprint Technique in Monitoring the ES Cell Chimeric Mice

TANG Fuchou,XUE Youfang,LI Meiqin,SHANG Kegang   

  1. Laboratory of Genetics, College of Life Science, Peking University, Beijing, 100871
  • Received:1999-12-02 Online:2000-11-20 Published:2000-11-20

摘要: 尝试应用多位点DNA指纹技术,检测经过胚胎干细胞(ES细胞)途径所获得的嵌合体小鼠中ES细胞在各种脏器中的嵌合情况、检测ES细胞在嵌合体小鼠中是否实现种系传递。结果表明:采用新型的人工合成的寡核苷酸多聚体探针(JL-02探针)的多位点DNA指纹图谱,具有足够的多态性和很好的稳定性。适用于鉴定ES细胞在嵌合体小鼠各种组织器官中的嵌合情况,也适用于鉴定ES细胞是否具有种系嵌合能力,尤其适用于在嵌合体研究中,最适宜的品种组合具有相同的毛色或相同的酶型。

关键词: 小鼠, 限制性片段长度多态性, DNA指纹图, JL-02探针, 胚胎干细胞

Abstract: Multi-locus DNA fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting ES cells into blastocysts and to detect whether the chimeric mouse is a germ-line chimeras. The results indicated that: The multi-locus DNA fingerprint with a new synthesized probe-JL-02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of ES cell chimeric mouse and to check whether an ES cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers.

Key words: mouse, restriction fragment length polymorphism, DNA fingerprint, probe JL-02, ES cell

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