曼氏血吸虫28kDa谷胱甘肽S-转移酶的有效抗原肽P141结构与活性研究

北京大学学报（自然科学版）

曼氏血吸虫28kDa谷胱甘肽S-转移酶的有效抗原肽P141结构与活性研究

许家喜¹,秦致辉²,易有云²,蔡孟深²

¹北京大学化学与分子工程学院，北京，100871；²北京医科大学药学院，北京，100083

收稿日期:1997-11-25 出版日期:1999-03-20 发布日期:1999-03-20

Studies on Structure and Activity of Effective Antigenic Peptide P141 of 28kDa Glutathione S-Transferase of Schistosoma Mansoni

XU Jiaxi¹, QIN Zhihui², YI Youyun², CAI Mengshen²

¹College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871; ²School of Phamarceutical Sciences, Beijing Medical University, Beijing, 100083

Received:1997-11-25 Online:1999-03-20 Published:1999-03-20

摘要/Abstract

摘要： 以曼氏血吸虫28kDa谷胱甘肽S-转移酶的有效抗原肽P141为先导肽，通过分别改变在结构上、Chou-Fasman构象参数上和亲水性上相似的氨基酸残基设计并合成了4个类似物，通过ELISA法测定了它们对血吸虫表膜抗原多克隆抗体的抗原性，通过类似物与P141的活性比较讨论了构效关系。

关键词: 曼氏血吸虫, 谷胱甘肽S-转移酶, 抗原肽, 构效关系

Abstract: Four analogs of effective antigenic peptide P141 of 28kDa glutathione S-transferase of schistosoma mansoni have been designed according to similarity of the amino acid residues in the structure, Chou-Fasman's secondary structural parameters P_α, P_β and P_t values and hydrophilicity of amino acid residues, respectively. They were synthesized by using solid phase method and their antigenicities were assayed by ELISA method with anti-schistosomal polyclonal antibodies to surface membrane antigen. The structure-activity relationship of P141 and its analogs was discussed.

Key words: Schistosoma mansoni, Glutathione S-transferase, Antigenic peptide, structureactivity relationship

中图分类号:

Q516

许家喜,秦致辉,易有云,蔡孟深. 曼氏血吸虫28kDa谷胱甘肽S-转移酶的有效抗原肽P141结构与活性研究[J]. 北京大学学报（自然科学版）.

XU Jiaxi,QIN Zhihui,YI Youyun,CAI Mengshen. Studies on Structure and Activity of Effective Antigenic Peptide P141 of 28kDa Glutathione S-Transferase of Schistosoma Mansoni[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

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