ChelexR100提取果蝇非新鲜标本核基因组方法的建立

北京大学学报（自然科学版）

Chelex^R100提取果蝇非新鲜标本核基因组方法的建立

马云梅, 吕静, 戴灼华

北京大学生命科学学院，北京，100871

收稿日期:1997-08-16 出版日期:1998-11-20 发布日期:1998-11-20

The Foundation of Chelex^R100 Method to Extract the Genome of Drosophila Non-Fresh Materials

MA Yunmei, LU Jing, DAI Zhuohua

College of Life Sciences, Peking University, Beijing, 100871

Received:1997-08-16 Online:1998-11-20 Published:1998-11-20

摘要/Abstract

关键词: 果蝇, 非新鲜标本, rRNA基因, ITS1

Abstract: The genome of Drosophila immigrans non-fresh materials (soaked in 70% & 100% ethanoland dried materials) was extracted with Chelex^R100. Then the ITS1 fragment which is between 18S rRNA and 5.8S rRNA was amplified with primers designed by our laboratory. The amplified fragment was linked to pGEM-T easy Vector (Promega company) and the final plasmid was transformed to E.coli DH5a. Then the plasmid was extracted and digested withendonuclease. According to the result of one of the non-fresh materials, the effects that templates were prepared using the Chelex method and the usual method were the same. Furthermore, the pollution and DNA waste were reduced in the Chelex method.

Key words: Drosophila immgrans, non-fresh materials, rRNA gene, ITS1

中图分类号:

Q349.13

马云梅, 吕静, 戴灼华. Chelex^R100提取果蝇非新鲜标本核基因组方法的建立[J]. 北京大学学报（自然科学版）.

MA Yunmei,LU Jing,DAI Zhuohua. The Foundation of Chelex^R100 Method to Extract the Genome of Drosophila Non-Fresh Materials[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

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链接本文: https://xbna.pku.edu.cn/CN/

https://xbna.pku.edu.cn/CN/Y1998/V34/I6/841

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