抗大鼠肝脏脂酶抗血清的制备及应用

北京大学学报（自然科学版）

抗大鼠肝脏脂酶抗血清的制备及应用

余瑞元¹, EBERT D L²

¹北京大学生命科学学院，北京，100871; ²澳大利亚墨尔本大学，墨尔本

收稿日期:1997-07-04 出版日期:1998-11-20 发布日期:1998-11-20

Preparation and use of Antisera Against Rat Hepatic Lipase

YU Ruiyuan¹, EBERT D L²

¹College of Life Science, Peking University, Beijing, 100871; ²University of Melbourne, Australia

Received:1997-07-04 Online:1998-11-20 Published:1998-11-20

摘要/Abstract

摘要： 按照RHL cDNA核苷酸序列所预示肽链的氨基酸序列，化学合成了与肽链N端、C端和中部相对应的3个肽段HL1、HL2和HL3，并将它们分别偶联上载体蛋白KLH，以偶联蛋白为免疫原免疫家兔，获得了抗血清。经ELISA检测表明，抗血清的滴度为1：51200～102400。用所制备的抗血清进行Western印迹分析，成功地检测了大鼠肝脏脂酶及其基因表达产物。

关键词: 合成肽, 大鼠肝脏脂酶, Western印迹分析, 抗血清, 表达产物

Abstract: According to rat hepatic lipase (RHL) cDNA nucleotide sequence and the predicted amino acid sequence, 3 polypeptides that correspond to amino, carboryl termini and middle part of RHL were chemically synthesized, coupled to carrierprotein keyhole limpet hemocyanin(KLH) respectively and then used as immunogens in rabbits. Antisera were raised and the titer (1:51200-102400) were detectable by ELISA. The antisera were applied to check the existing of RHL and their gene expression product by western blotting.

Key words: synthetic polypeptide, rat hepatic lipase, Western blotting, antisera, expression product

中图分类号:

Q511

余瑞元, EBERT D L. 抗大鼠肝脏脂酶抗血清的制备及应用[J]. 北京大学学报（自然科学版）.

YU Ruiyuan,EBERT D L. Preparation and use of Antisera Against Rat Hepatic Lipase[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://xbna.pku.edu.cn/CN/

https://xbna.pku.edu.cn/CN/Y1998/V34/I6/759

[1]	孙雷,黄仪秀,朱圣庚. 噬菌体单链抗体导向溶栓剂的构建[J]. 北京大学学报（自然科学版）, 2000, 36(6): 881-884.
[2]	高新胜, 黄仪秀, 郭振泉, 朱圣庚, 陈玉珍. 小鼠噬菌体抗体库的构建和N-肽结合单链抗体的筛选[J]. 北京大学学报（自然科学版）, 2000, 36(4): 489-494.