PET纤维上SuMA的紫外光接枝及对蛋白质的固定化

北京大学学报（自然科学版）

PET纤维上SuMA的紫外光接枝及对蛋白质的固定化

李军, 伊敏, 刘宁, 哈鸿飞

北京大学技术物理学系，北京，100871

收稿日期:1997-09-10 出版日期:1998-11-20 发布日期:1998-11-20

The UV Induced Grafting of SuMA onto PET Fiber and Its Application of Immobilization of Protein

LI Jun, YI Min, LIU Ning, HA Hongfei

Department of Technical Physics, Peking University, Beijing, 100871

Received:1997-09-10 Online:1998-11-20 Published:1998-11-20

摘要/Abstract

摘要： 通过使用NaIO₄而不需除氧的条件下，利用紫外光接枝技术，成功地将SuMA或SuMA和HEMA二元单体共接枝到PET纤维上。在相同SuMA单体浓度下，HEMA的加入能显著提高SuMA的接枝率。如所期望的那样，接枝在PET纤维上的SuMA，在近中性的水溶液中，通过其活性酯基与蛋白质的—NH₂反应，成功实现了对胰蛋白酶和肝炎表面抗体的固定化；HEMA的加入虽然提高了SuMA的接枝率，但是HEMA接枝产生的空阻却显著减少了固定的蛋白质量，使其低于仅SuMA接枝的情况。

关键词: SuMA, 紫外光接枝, 胰蛋白酶, 肝炎表面抗体, 固定化

Abstract: The monomer of SuMA or SuMA and HEMA was grafted onto the PET fibers successfully under UV irradiation by the use of NaIO₄ instead of the degassing process. Under the same concentration of SuMA, the use of HEMA could enhance the grafting yield of SuMA onto PET fibers obviously comparing with the process which only SuMA was used. As expected, proteins of trypsin and hepatitis surface antibody were immobilized onto the SuMA grafted PET fibers successfully under the neutral aqueous solutions, which was realized through the reaction between the active ester group of SuMA and amino group of protein. Although the use of HEMA during UV grafting could improve the grafting yield of SuMA onto PET fibers, the special impede on the couple reaction of SuMA and proteins caused by the grafted HEMA on PET remarkably inhibited the immobilization of protein, made the amount of immobilized protein lower than that of the only SuMA grafted samples.

Key words: SuMA, UV grafting, trypsin, hepatitis surface antibody, immobilization

中图分类号:

O647

李军, 伊敏, 刘宁, 哈鸿飞. PET纤维上SuMA的紫外光接枝及对蛋白质的固定化[J]. 北京大学学报（自然科学版）.

LI Jun,YI Min,LIU Ning,HA Hongfei. The UV Induced Grafting of SuMA onto PET Fiber and Its Application of Immobilization of Protein[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://xbna.pku.edu.cn/CN/

https://xbna.pku.edu.cn/CN/Y1998/V34/I6/746

[1]	杨光,张剑波,王杉霖,杨宇翔,宗悦茹,王维敬. 固定化过氧化物酶催化去除五氯酚反应装置和条件探讨[J]. 北京大学学报（自然科学版）, 2008, 44(2): 233-236.
[2]	李晶,倪晋仁,籍国东. 填充大孔载体的生物滴滤塔脱除H₂S的研究[J]. 北京大学学报（自然科学版）, 2007, 43(4): 572-577.
[3]	陈嵩,张剑波,陈辉,杨宇翔. 废水中铁离子对漆酶催化降解2,4-二氯苯酚的影响[J]. 北京大学学报（自然科学版）, 2006, 42(4): 490-495.
[4]	陈辉,张剑波,刘小鹏,杨宇翔. 漆酶催化降解氯酚类有机污染物[J]. 北京大学学报（自然科学版）, 2005, 41(4): 605-611.
[5]	唐建国, 茹炳根, 徐长法, 胡美浩, 朱圣庚. 蛋白质工程的研究[J]. 北京大学学报（自然科学版）, 1998, 34(2): 342-349.
[6]	唐建国, 茹炳根, 徐长法, 胡美浩, 朱圣庚. 蛋白质工程的研究[J]. 北京大学学报（自然科学版）, 1998, 34(2): 342-349.
[7]	王瑛,陈来同 . 基因拷贝数分析的研究[J]. 北京大学学报（自然科学版）, 1996, 32(6): 781-784.