关键词: 移植免疫, 供体特异性免疫耐受, 供体细胞胸腺注射, T细胞克隆流产, 蓖麻毒蛋白定向杀伤

Abstract: The way to obtain the prolonged donor-specific tolerance after skin allograft must include both the specific lysis of the immunocompetent cells in the peripheral blood and lymphoid, and the induction of the recognition as self-antigen in the central immunological organ. The adult BALB/C mouse recipient was tail-vein injected with C₅₇BL/6 donor splenocytes loaded ricin, a kind of plant toxin. The guided toxic cells selectively killed the T, Blymphocytes which could specifically recognize the allografted antigens on the surface of C₅₇BL/6 cells. In advance, the recipient had been given C₅₇BL/6 splenocytes via the intrathymic route to achieve clone deletion of alloreactive T cells during development. These treatments induced a donorspecific tolerance which allowed the skin grafts on the BALB/C mice to survive prolongedly without the need for further immunosuppression (mean survival time[MST]>60 days, control group's MST=5.0 days). The results of MTT colorimetric assay after one-way mixed lymphocytes co-culture provided the cellular evidence in vitro.

Key words: immunity to transplantation, donor-specific tolerance, donor cell intrathymic injection, T cell cloned deletion, ricin directed lysis