关键词: 黄粉甲幼虫, 血细胞总数, 杀菌率, 体液防卫, 诱导期

Abstract: The hemolymph defence responses of the larvae in last instar of Tenerio moliter were experimentally studied. The results were that: (1) When the larvae were stimulated by a needle and injected with Escherchia coli, Bacillus thuringiensis and sterile saline severally, the total hemocytes count (THC) in all treatments changed regularly in the similar way: first, THC reduced sharply in 15 min postinjection, then three peaks of THC occured within 10h postinjection, but the scope of the change was different. (2) The remnant quantity of bacteria in the larvae reduced gradually when the larvae were injected with the two kinds of bacteria. The peak of THC accompanied with a peak of disinfection. The peak of the rate of relative disinfection occured in 1～2h and 4～6h postinjection. (3) The inducement period of humoral defence of the larvae was 6～8h postinjection, so the cellular reaction occured in the early to microbial invaders and humoral defence reaction participated in 6～8h postinjection.

Key words: Tenebrio moliter larvae, total hemocytes count, ratios of disinfection, inducement period of humoral defence