关键词: 表面展示, 结构库, 靶功能多肽, 亲和选择, 蛋白质的改造, 表面展示, 结构库, 靶功能多肽, 亲和选择, 蛋白质的改造

Abstract: Surface display is a newly developed gene manipulation technology by which fragments of foreign peptides are expressed as fusion proteins displayed on the phage or cell surface, keeping their spatial structure and biological activity relatively independent. This technology may be used for studying the properties, mutual recognition and interaction of peptides (proteins), so as to survey very large display libraries for target functional peptides. There are three display systems, including filamentous bacteriophage, T₄ and T₇ phage, and cell surface systems, mosthy used so far. Display libraries include recombinant phage antibody libraries, random short peptide libraries, peptide comformation libraries, cDNA display libraries, and gene mutant display libraries. The surface display technology can be used for the production of artificial antibody and vaccine, the determination of antigen epitopes and protein interactive sites, the isolation of regulatory molecules, the study of cell surface engineering, the development of peptide drugs, and the experimentally directed evolution of biomelecules.

Key words: protein transformation, surface display, structural library, target functional peptide, affinity selection, surface display, structural library, target functional peptide, affinity selection, protein transformation