关键词: 移植免疫, 免疫耐受, 供体细胞介导的免疫抑制, 蓖麻毒蛋白

Abstract: A new approach for preventing rejection of allografts is described. The donor of skin allograft is C₅₇BL/6, and the receptor is BALB/C. We used calcium chloride to transduct ricin into donor spleen cells (getting Toxic Cell's), and tail-vein injected into receptors blood. Because Toxic Cell's have intact transplantation antigen (Major Histocompatible Antigen Complex, MHC) on their surface, they can be specifically recognized and lysed by receptor's T, B lymphocytes. After being lysed, Toxic Cell's would release ricin to kill the specific receptor's T, B lymphocytes, and finally induce unresponsiveness towards allografts. There were two test groups, and they were all injected with Toxic Cell's after or before skin transplantation. The survival time of skin allografts in both test groups were prolonged compared with respective control groups. The results of statistic assay ( t-test) also verified that the difference between each pair of test and control groups was very significant (t＞t_0.01). Mixed lymphocyte culture test had also substantiated that the immune response to donor MHC of both test groups had been suppressed by our new method.

Key words: immunity to transplantation, immunotolerance, immunosuppression induced by donor cells, ricin