6个小鼠ES细胞系的建立和鉴定

北京大学学报（自然科学版）

6个小鼠ES细胞系的建立和鉴定

胡新立, 尚克刚

北京大学生命科学学院，北京，100871

收稿日期:1995-04-21 出版日期:1996-03-20 发布日期:1996-03-20

Establishment and Characterization of Six ES Cell Lines from Mouse 129/ter Strain

HU Xinli, SHANG Kegang

College of Life Science, Peking University, Beijing, 100871

Received:1995-04-21 Online:1996-03-20 Published:1996-03-20

摘要/Abstract

摘要： 报道从小鼠129/ter品系中建成6个胚胎干细胞系，经过核型检查，体内、体外分化等鉴定后发现，6个细胞系中有2个细胞系是正常二倍体，其中ES细胞系MESPU 13为XY型，其核骨架蛋白质成分相当于3～4d(天)的鼠胚细胞的核骨架成分，可长期维持二倍体未分化状态，并具有高效的嵌合能力。核型不正常的细胞系所形成的畸胎瘤生长较快，但细胞的分化程度与核型正常的ES细胞有所不同。对影响建系结果的各种因素进行了讨论。

关键词: 小鼠, ES细胞系, 细胞分化

Abstract: Six mouse Smbryonic stem cell lines were established from strain 129/ter.Their karyotypes as well as their differentiation ability in vivo and in vitro had been tested. Two of the six cell lines had normal diploid karyotypes, and MESPU 13 was a XY ES cell line. MESPU 13 could keep its normal karyotype and undifferentiated state over long period of culture, and it was highly efficient in forming chimeras. The cell skeleton protein content of MESPU 13 was the same as that of the 3--4 days old murin embryonic cells. The teratomas of the karyotypically abnormal ES cell lines grew faster,but their differentiation extent was slightly different from those of the normal ones. The factors which might affect the establishment of ES cell lines had also been discussed.

Key words: mouse, ES cell lines, cell differentiation

中图分类号:

Q953.5

胡新立, 尚克刚 . 6个小鼠ES细胞系的建立和鉴定[J]. 北京大学学报（自然科学版）.

HU Xinli,SHANG Kegang. Establishment and Characterization of Six ES Cell Lines from Mouse 129/ter Strain[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://xbna.pku.edu.cn/CN/

https://xbna.pku.edu.cn/CN/Y1996/V32/I2/248

[1]	滕春波, 宋晓明, 马红, 杨增明. 促性腺激素处理对性未成熟小鼠子宫中STAT3表达和激活的影响[J]. 北京大学学报（自然科学版）, 2004, 40(4): 565-572.
[2]	汤富酬,薛友纺,李美琴,尚克刚. DNA指纹图在小鼠ES细胞嵌合体鉴定中的应用[J]. 北京大学学报（自然科学版）, 2000, 36(6): 789-794.
[3]	童英, 邹冀中, 尚克刚. 用大鼠心肌条件培养基建立来源于C57BL/6J小鼠的ES细胞系[J]. 北京大学学报（自然科学版）, 2000, 36(4): 472-476.
[4]	童英, 刘爱民, 尚克刚, 刘林. 兔ES样细胞系的建立及其特性分析[J]. 北京大学学报（自然科学版）, 1997, 33(4): 500-507.
[5]	韩嵘, 柴桂萱, 尚克刚. 大鼠心肌条件培养基对形成小鼠ES细胞集落的影响[J]. 北京大学学报（自然科学版）, 1997, 33(2): 185-188.
[6]	周未艾, 高天礼 . 小鼠心脏缺灌和再灌早期乳酸脱氢酶释放规律[J]. 北京大学学报（自然科学版）, 1996, 32(2): 239-247.