关键词: 百合鳞片, 离体形态发生, 同工酶

Abstract: The bulb scales of Lilium were used as explants and cultured on MS basic medium supplemented with 1 mg/L IBA and 0.5 mg/L 6-BA to build up an experimental system for studying organogenesis in vitro. At about the 10th day of culturing, bulblets grew out from adaxial surfaces of the bulb-scales. The induction rate was 100%. Specimens were taken everyday since the 1st day of culturing for cytohistological observations and the extractions of proteins. The extracts analysed by isoelectrofocusing polyacrylamide gel electrophoresis (IEF-PAGE). The results were as follows: The amount of total protein kept stable in the first two days and decreased to the lowest at the fifth day of culture, and then increased, but decreased again from the seventh day. There were changes of proteins with pI between 6.2 and 6.6. Both the composition and the amount of each component changed. During the culture of Lilium bulb scales in vitro, both the activity of peroxidase isozymes and the kinds of peroxidase isozymes increased gradually. Four distinct bands appeared in the cathode zone. The amounts of esterase isozymes with pI4.6～5.9 and 6.6～8.2increased. The amount and the activity of amylase isozymes also increased gradually. These results suggested that a further investigation using this system could lead to a better understanding of the isozyme changes in organogenesis.

Key words: bulb scales of Lilium davidii, organogenesis, isozyme