固相萃取－高效液相色谱法测定对虾体内土霉素残留量

北京大学学报（自然科学版）

固相萃取－高效液相色谱法测定对虾体内土霉素残留量

卜一工,赵彤,侯艳文,朱涛,刘虎威,孙亦梁

北京大学化学系，北京，100871

收稿日期:1993-12-09 出版日期:1995-07-20 发布日期:1995-07-20

Determination of Oxytetracycline Residue in Shrimps by Solid Phase Extraction-High Performance Liquid Chromatography

BU Yigong, ZHAO Tong, HOU Yanwen, Zhu Tao, LIU Huwei, SUN Yiliang

Department of Chemistry, Peking University, Beijing, 100871

Received:1993-12-09 Online:1995-07-20 Published:1995-07-20

摘要/Abstract

摘要： 用绞碎机破碎对虾样品，加入0.1mol/L EDTA-Mcllvaine缓冲液匀浆，匀浆液离心后，上层清液加入NaCl脱蛋白，再次离心，过滤，滤液经Sep-pak C₁₈固相萃取小柱富集后，用高效液相色谱法分析。

关键词: 对虾, 固相萃取, 高效液相色谱, 土霉素残留量

Abstract: A shrimp sample was blended to small pieces and homogenized with 0.1 mol/L EDTA-McIlvaine buffer. The homogenates were centrifuged and sodium chloride was added into the supernatant to precipitate the proteins. After centrifugation and filtration, the filtrate was applied on the sep-pak C₁₈ cartridge. An aliquot of 25 micro-liter was injectedinto HPLC with CN bonded phase as column packings.

Key words: shrimp, solid phase extraction, high performance liquid chromatography, oxytetracycline residue

中图分类号:

O657.72

卜一工,赵彤,侯艳文,朱涛,刘虎威,孙亦梁. 固相萃取－高效液相色谱法测定对虾体内土霉素残留量[J]. 北京大学学报（自然科学版）.

BU Yigong,ZHAO Tong,HOU Yanwen,Zhu Tao,LIU Huwei,SUN Yiliang. Determination of Oxytetracycline Residue in Shrimps by Solid Phase Extraction-High Performance Liquid Chromatography[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

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https://xbna.pku.edu.cn/CN/Y1995/V31/I4/415