通过二（半胱氨酰）赖氨酸用99mTc标记生物素

北京大学学报（自然科学版）

通过二（半胱氨酰）赖氨酸用^99mTc标记生物素

董燕兵,吴永慧,刘斌,刘元方

北京大学技术物理系，北京，100871

收稿日期:1993-08-26 出版日期:1995-03-20 发布日期:1995-03-20

Labeling Biotin with Tc-99m via N, N'-Biscysteinyl-Lysine

DONG Yanbing, WU Yonghui, LIU Bin, LIU Yuanfang

Department of Technical Physics, Peking University, Beijing, 100871

Received:1993-08-26 Online:1995-03-20 Published:1995-03-20

摘要/Abstract

摘要： 生物素（biotin）和亲和素（avidin）具有很强的亲和力，这一特性可以被用于改进放免显像的预定位技术。本文阐述了利用一种新的含硫含氮配体L-N-N′-二（半胱氨酰）赖氨酸（BCL）作为联接剂，用^99mTc标记生物素的方法。初步研究的结果表明，标记物具有较好的体内外稳定性，可以进一步用于抗肿瘤单抗预定位，再注射能与亲和素结合的放标生物素进行肿瘤显像。

关键词: 放免显像, 预定位技术, 二（半胱氨酰）赖氨酸, 生物素－亲和素

Abstract: A new biotin derivative was systhesized by conjugatingN, N'-biscysteinyl-lysine(BCL) to biocytin. The conditions of conjugationwas studied BCL-biocytine was labeled with Tc-99m. The labels was identified by TLC, GC and bonding with avidin. The biodistribution of the labels in normal mice was also investigated. The stability of Tc-99m-BCL-biocytine both in vitro and in vivo suggested that the labels may be further used in pretargeting.

Key words: immunoimaging, pretargeting, N, N'-biscysteinyl-lysine, avidin-biotin

中图分类号:

R817

董燕兵,吴永慧,刘斌,刘元方. 通过二（半胱氨酰）赖氨酸用^99mTc标记生物素[J]. 北京大学学报（自然科学版）.

DONG Yanbing,WU Yonghui,LIU Bin,LIU Yuanfang. Labeling Biotin with Tc-99m via N, N'-Biscysteinyl-Lysine[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

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https://xbna.pku.edu.cn/CN/Y1995/V31/I2/198