北京大学学报(自然科学版) ›› 2015, Vol. 51 ›› Issue (4): 667-676.DOI: 10.13209/j.0479-8023.2015.092

• 研究论文 • 上一篇    下一篇

真菌对重金属Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ)和Cr(Ⅵ)耐受性的比较研究

杨振兴;田从魁;党晨原;常方;倪晋仁   

  1. 北京大学环境科学与工程学院, 水沙科学教育部重点实验室, 北京100871;
  • 收稿日期:2014-04-03 修回日期:2014-06-20 出版日期:2015-07-20 发布日期:2015-07-20
  • 倪晋仁
  • 基金资助:
    国家水体污染控制与治理科技重大专项(2010ZX07212-008)资助

Comparative Study on Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ), Cr(Ⅵ) Resistance Characteristics of Fungus

YANG Zhenxing;TIAN Congkui;DANG Chenyuan;CHANG Fang;NI Jinren   

  1. College of Environmental Sciences and Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences (MOE), Beijing 100871;
  • Received:2014-04-03 Revised:2014-06-20 Online:2015-07-20 Published:2015-07-20
  • About author:NI Jinren

摘要: 从两种不同土壤环境中筛选获得19 株真菌, 并对其重金属耐受性进行比较分析。其中, 从重金属污染严重的湖南水口山有色金属矿区土壤中筛选获得17 株真菌, 经鉴定分别属于曲霉属、木霉属、青霉属和镰孢属; 从重金属污染较轻的廊坊经济开发区绿化用地中筛选获得2 株真菌, 经鉴定均为黑曲霉。研究重金属对不同土壤环境中分离的真菌的最小抑制浓度(MICs), 分析菌种、生存环境、重金属浓度等因素对真菌重金属耐受性的影响, 探讨真菌耐受高浓度重金属的机理。研究表明, 从矿区分离获得的真菌中, Aspergillus niger PTN84 等可耐受144 mmol/L Pb(Ⅱ), A. terreus PTN21, A. flavus PTN29 和Trichoderma asperellum PTN1 可耐受36 mmol/L Cd(Ⅱ), Fusarium sp. PTN12, A. terreus PTN21 和T. asperellum PTN1 可耐受36 mmol/L Cu(Ⅱ), Fusarium sp. PTN12 可耐受72 mmol/L As(Ⅲ), 整体上其重金属耐受能力高于文献报道的菌株, 在重金属土壤修复方面具有潜在的应用价值。

关键词: 真菌, 重金属耐受性, 最小抑制浓度

Abstract: 19 fungus were isolated from two different soil environments and a comparative analysis of their heavy metal tolerance was conducted. Among them, 18 fungus, i.e. Aspergillus sp., Trichoderma sp., Penicillium sp., Fusarium sp., were isolated respectively from the contaminated soil of Shuikoushan nonferrous metals mine in Hunan Province; 2 fungus, identified as Aspergillus niger., were isolated from non-contaminated soil in green land located in Langfang Economic Development Zone. Fungus isolated from the contaminated soil and noncontaminated green land as well as those reported previously are compared in terms of their heavy metal resistance characterized with the minimal inhibitory concentrations (MICs). As results, mechanism of fungal resistance of high concentrations of heavy metals was revealed. The fungus of heavy metal resistance primarily relies on the sites of their isolation, metal species, fungi species and metal speciation. Considering their high heavy-metal tolerance, e.g. MICs 144 mmol/L for Pb(Ⅱ), 36 mmol/L for Cd(Ⅱ) and Cu(Ⅱ), and 72 mmol/L for As(Ⅲ), the isolated fungus perform excellent and thus have higher potential for soil restoration.

Key words: fungi, resistance to heavy metal, MICs

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