真菌对重金属Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ)和Cr(Ⅵ)耐受性的比较研究

doi:10.13209/j.0479-8023.2015.092

北京大学学报（自然科学版） ›› 2015, Vol. 51 ›› Issue (4): 667-676.DOI: 10.13209/j.0479-8023.2015.092

真菌对重金属Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ)和Cr(Ⅵ)耐受性的比较研究

杨振兴;田从魁;党晨原;常方;倪晋仁

北京大学环境科学与工程学院, 水沙科学教育部重点实验室, 北京100871;

收稿日期:2014-04-03 修回日期:2014-06-20 出版日期:2015-07-20 发布日期:2015-07-20
倪晋仁
基金资助:
国家水体污染控制与治理科技重大专项(2010ZX07212-008)资助

Comparative Study on Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ), Cr(Ⅵ) Resistance Characteristics of Fungus

YANG Zhenxing;TIAN Congkui;DANG Chenyuan;CHANG Fang;NI Jinren

College of Environmental Sciences and Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences (MOE), Beijing 100871;

Received:2014-04-03 Revised:2014-06-20 Online:2015-07-20 Published:2015-07-20
About author:NI Jinren

摘要/Abstract

摘要： 从两种不同土壤环境中筛选获得19 株真菌, 并对其重金属耐受性进行比较分析。其中, 从重金属污染严重的湖南水口山有色金属矿区土壤中筛选获得17 株真菌, 经鉴定分别属于曲霉属、木霉属、青霉属和镰孢属; 从重金属污染较轻的廊坊经济开发区绿化用地中筛选获得2 株真菌, 经鉴定均为黑曲霉。研究重金属对不同土壤环境中分离的真菌的最小抑制浓度(MICs), 分析菌种、生存环境、重金属浓度等因素对真菌重金属耐受性的影响, 探讨真菌耐受高浓度重金属的机理。研究表明, 从矿区分离获得的真菌中, Aspergillus niger PTN84 等可耐受144 mmol/L Pb(Ⅱ), A. terreus PTN21, A. flavus PTN29 和Trichoderma asperellum PTN1 可耐受36 mmol/L Cd(Ⅱ), Fusarium sp. PTN12, A. terreus PTN21 和T. asperellum PTN1 可耐受36 mmol/L Cu(Ⅱ), Fusarium sp. PTN12 可耐受72 mmol/L As(Ⅲ), 整体上其重金属耐受能力高于文献报道的菌株, 在重金属土壤修复方面具有潜在的应用价值。

关键词: 真菌, 重金属耐受性, 最小抑制浓度

Abstract: 19 fungus were isolated from two different soil environments and a comparative analysis of their heavy metal tolerance was conducted. Among them, 18 fungus, i.e. Aspergillus sp., Trichoderma sp., Penicillium sp., Fusarium sp., were isolated respectively from the contaminated soil of Shuikoushan nonferrous metals mine in Hunan Province; 2 fungus, identified as Aspergillus niger., were isolated from non-contaminated soil in green land located in Langfang Economic Development Zone. Fungus isolated from the contaminated soil and noncontaminated green land as well as those reported previously are compared in terms of their heavy metal resistance characterized with the minimal inhibitory concentrations (MICs). As results, mechanism of fungal resistance of high concentrations of heavy metals was revealed. The fungus of heavy metal resistance primarily relies on the sites of their isolation, metal species, fungi species and metal speciation. Considering their high heavy-metal tolerance, e.g. MICs 144 mmol/L for Pb(Ⅱ), 36 mmol/L for Cd(Ⅱ) and Cu(Ⅱ), and 72 mmol/L for As(Ⅲ), the isolated fungus perform excellent and thus have higher potential for soil restoration.

Key words: fungi, resistance to heavy metal, MICs

中图分类号:

X172

杨振兴;田从魁;党晨原;常方;倪晋仁. 真菌对重金属Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ)和Cr(Ⅵ)耐受性的比较研究[J]. 北京大学学报（自然科学版）, 2015, 51(4): 667-676.

YANG Zhenxing;TIAN Congkui;DANG Chenyuan;CHANG Fang;NI Jinren. Comparative Study on Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ), Cr(Ⅵ) Resistance Characteristics of Fungus[J]. , 2015, 51(4): 667-676.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://xbna.pku.edu.cn/CN/10.13209/j.0479-8023.2015.092

https://xbna.pku.edu.cn/CN/Y2015/V51/I4/667

参考文献

[1] Amini M, Younesi H, Bahramifar N, et al. Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergillus niger. Journal of Hazardous Materials, 2008, 154: 694–702
[2] Jordan M J, Lechevalier M P. Effects of zinc-smelter emissions on forest soil microflora. Canadian Journal of Microbiology, 1975, 21: 1855–1865
[3] Pennanen T, Frostegard A, Fritze H, et al. Phospholipid fatty acid composition and heavy metal tolerance of soil microbial communities along two heavy metal-polluted gradients in coniferous forests. Applied and Environmental Microbiology, 1996, 62: 420–428
[4] Anand P, Isar J, Saran S, et al. Bioaccumulation of copper by Trichoderma viride. Bioresource Technology, 2006, 97: 1018–1025
[5] Islam M, Saha A, Mosaddeque H, et al. In vitro studies on the reaction on fungi Trichoderma to different herbicides used in tea plantation. Int J Sustain Crop Prod, 2008, 3: 27–30
[6] Zafar S, Aqil F, Ahmad I. Metal tolerance and biosorption potential of filamentous fungi isolated from metal contaminated agricultural soil. Bioresource Technology, 2007, 98: 2557–2561
[7] Murray M, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 1980, 8: 4321–4326
[8] Muñoz A J, Ruiz E, Abriouel H, et al. Heavy metal tolerance of microorganisms isolated from wastewaters: identification and evaluation of its potential for biosorption. Chemical Engineering Journal, 2012, 210: 325–332
[9] Ezzouhri L, Castro E, Moya M, et al. Heavy metal tolerance of filamentous fungi isolated from polluted sites in Tangier, Morocco. African Journal of Microbiology Research, 2009, 3: 35–48
[10] Iskandar N L, Zainudin N A I M, Tan S G. Tolerance and biosorption of copper (Cu) and lead (Pb) by filamentous fungi isolated from a freshwater ecosystem. Journal of Environmental Sciences, 2011, 23: 824–830
[11] Iram S, Zaman A, Iqbal Z, et al. Heavy metal tolerance of fungus isolated from soil contaminated with sewage and industrial wastewater. Polish Journal of Environmental Studies, 2013, 22: 691–697
[12] Iram S, Waqar K, Shuja N, et al. Tolerance potential of different species of Aspergillus as bioremediation tool — comparative analysis. E3 Journal of Microbiology Research, 2011, 1: 001–008
[13] Çolak F, Atar N, Yaz?c?o?lu D, et al. Biosorption of lead from aqueous solutions by Bacillus strains possessing heavy-metal resistance. Chemical Engineering Journal, 2011, 173: 422–428
[14] Castro-Silva M A, Lima A O D S, Gerchenski A V, et al. Heavy metal resistance of microorganisms isolated from coal mining environments of Santa Catarina. Brazilian Journal of Microbiology, 2003, 34: 45–47 北京大学学报(自然科学版) 第51 卷第4 期 2015 年7 月 676
[15] Rossbach S, Wilson T L, Kukuk M L, et al. Elevated zinc induces siderophore biosynthesis genes and a zntA-like gene in Pseudomonas fluorescens. FEMS Microbiology Letters, 2000, 191: 61–70
[16] Tam P C. Heavy metal tolerance by ectomycorrhizal fungi and metal amelioration by Pisolithus tinctorius. Mycorrhiza, 1995, 5: 181–187
[17] Vadkertiová R, Sláviková E. Metal tolerance of yeasts isolated from water, soil and plant environments. Journal of Basic Microbiology, 2006, 46: 145–152
[18] Cánovas D, Durán C, Rodríguez N, et al. Testing the limits of biological tolerance to arsenic in a fungus isolated from the River Tinto. Environmental Microbiology, 2003, 5: 133–138
[19] Banerjee S, Datta S, Chattyopadhyay D, et al. Arsenic accumulating and transforming bacteria isolated from contaminated soil for potential use in bioremediation. Journal of Environmental Science and Health, Part A, 2011, 46: 1736–1747
[20] Nies D H. Microbial heavy-metal resistance. Applied Microbiology and Biotechnology, 1999, 51: 730–750
[21] Cernansky S, Urík M, Ševc J, et al. Biosorption of arsenic and cadmium from aqueous solutions. African Journal of Biotechnology, 2007, 6: 1932–1934
[22] Valix M, Loon L. Adaptive tolerance behaviour of fungi in heavy metals. Minerals Engineering, 2003, 16: 193–198
[23] 廖继佩, 林先贵, 曹志洪, 内外生菌根真菌对重金属的耐受性及机理. 土壤, 2003, 35(5): 370–377
[24] Galli U, Schüepp H, Brunold C. Heavy metal binding by mycorrhizal fungi. Physiologia Plantarum, 1994, 92: 364–368
[25] Harrison J J, Ceri H, Turner R J. Multimetal resistance and tolerance in microbial biofilms. Nature Reviews Microbiology, 2007, 5: 928–938
[26] 黄艺 , 陶澍. 外生菌根对欧洲赤松苗 (Pinus sylvestris) Cu, Zn 积累和分配的影响. 环境科学, 2000, 21(2): 1–6
[27] 焦芳婵, 毛雪, 李润植, 金属结合蛋白基因及其在清除重金属污染中的应用. 遗传, 2002, 24(1): 82–86
[28] Tyler G. Metals in sporophores of basidiomycetes. Transactions of the British Mycological Society, 1980, 74: 41–49
[29] Bakken L R, Olsen R A. Accumulation of radiocaesium in fungi. Canadian Journal of Microbiology, 1990, 36: 704–710
[30] Turnau K, Kottke I, Dexheimer J. Toxic element filtering in Rhizopogon roseolus/Pinus sylvestris mycorrhizas collected from calamine dumps. Mycological Research, 1996, 100: 16–22
[31] Väre H. Aluminium polyphosphate in the ectomycorrhizal fungus Suillus variegatus (Fr.) O. Kunze as revealed by energy dispersive spectrometry. New Phytologist, 1990, 116: 663–668
[32] Cromack K Jr, Sollins P, Graustein W C, et al. Calcium oxalate accumulation and soil weathering in mats of the hypogeous fungus Hysterangium crassum. Soil Biology and Biochemistry, 1979, 11: 463–468

真菌对重金属Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ)和Cr(Ⅵ)耐受性的比较研究

Comparative Study on Pb(Ⅱ), Cd(Ⅱ), As(Ⅲ), Cr(Ⅵ) Resistance Characteristics of Fungus

PDF

PDF (翻译版)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

留言

[1]	柳叶, 任悦, 高广磊, 丁国栋, 张英, 赵珮杉, 王家源. 毛乌素沙地樟子松人工林根内真菌网络动态特征及其对气候因子的响应[J]. 北京大学学报自然科学版, 2023, 59(3): 467-477.
[2]	魏晓帅, 郭米山, 高广磊, 任悦, 丁国栋, 张英. 呼伦贝尔沙地樟子松根内真菌群落结构与功能群特征[J]. 北京大学学报自然科学版, 2020, 56(4): 710-720.
[3]	冉喜玲, 刘雨, 田从魁, 朱婷婷, 宋雅楠, 莫月贤. 硒矿区土壤中真菌的分离培养及抗肿瘤活性筛选[J]. 北京大学学报自然科学版, 2017, 53(6): 1161-1164.
[4]	杨金水, 杨扬, 孙良明, 刘伟杰, 曾远, 邓春萍, 邢冠岚, 袁红莉. 铅锌矿区土壤真菌响应重金属污染的群落组成变化[J]. 北京大学学报自然科学版, 2017, 53(2): 387-396.
[5]	黄园园,OLBRECHT Luise,杨晓霞,贺金生. 养分添加对青藏高原高寒草甸丛枝菌根真菌的影响[J]. 北京大学学报（自然科学版）, 2014, 50(5): 911-918.
[6]	马彦荣,田从魁,倪晋仁. 污泥中真菌Penicillium lanosum NT-121的细胞毒活性产物研究[J]. 北京大学学报（自然科学版）, 2013, 49(6): 1118-1120.
[7]	高尚,马丽华,黄民生,陶芳,陈诚. 连续式白腐真菌膜生物反应器处理染料废水: 微生物群落结构及其与脱色效率的关系[J]. 北京大学学报（自然科学版）, 2010, 46(3): 401-406.