Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae

Abstract

Abstract: The psaC Gene of Synechococcus sp. PCC 7002 encodes the F_A/F_B aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae.Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

Key words: Cyanobacteria, psaC, Saccharomyces cerevisiae, transformation

摘要： 聚球蓝藻(Synechococcus sp.PCC 7002)的psaC是光系统I的F_A/F_B脱辅基蛋白基因，其分子量8.9kD蛋白中含有2个4Fe-4S中心。构建了psaC基因的表达载体pYES2-psaC，将表达载体转化到酵母(Saccharomyces cerevisiae)中，Southern杂交证明psaC基因已被转化，诱导培养后的蛋白电泳显示psaC基因获得高效表达。

关键词: 蓝藻, psaC, 酵母, 转化

CLC Number:

LI Bin,LI Tao,TONG Xuemei,WANG Donghui,ZHAO Jindong,WU Guangyao. Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

李斌,李涛,童雪梅,王东辉,赵进东,吴光耀. 蓝藻光合基因psaC在酵母中的转化和表达[J]. 北京大学学报（自然科学版）.

[1]	SHANG Yunyi, LI Zhilong, SUN Liyu, CHEN Qian. Microbial Nitrogen Transformation Key Processes and Its Influencing Factors in Water and Sediment of Xining Section of the Huangshui River [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2023, 59(1): 92-104.
[2]	LI Meijin, TANG Jie, Md. Mahfuzur R. Shah, Maurycy Daroch. Identification and Characterization of a New Species of Thermoleptolyngbya Isolated from Hot Springs in Sichuan [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2022, 58(4): 739-752.
[3]	CHEN Zhaohui, MA Yuan, JING Jiwu. Hardware Optimization and Evaluation for Crucial Modules of Lattice-Based Cryptography [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2021, 57(4): 595-604.
[4]	LI Liheng, LIANG Yuanmei, LI Meijin, CHEN Pengyu, XIAO Meng, CUI Yixuan, Maurycy Daroch. Thermophilic Cyanobacteria PKUAC-E542 Phycocyanin Heat Resistance and Effects of Different Light Conditions on Its Accumulation [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2021, 57(3): 529-535.
[5]	MENG Jijun, ZHU Lijun, WANG Qi, GUO Liren, ZHANG Weijia. Influence of Policy-Driven Land Use Transformation on Multifunctional Land Use in the Middle Reaches of the Heihe River Basin [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2020, 56(6): 1102-1112.
[6]	LI Kai, GONG Yiwei, LI Xingkang, ZHANG Yanting, Maurycy Daroch, JIN Peng. Effects of Different Light Qualities on the Growth of Thermophilic Cyanobacteria Synechococcus sp. PCC6715 [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2020, 56(4): 739-744.
[7]	SU Yue, YANG Zhentao, YANG Bo, ZHAO Liang. Application Research of Active Source Rayleigh Wave Multi-Mode Extraction Method Based on Vector Wavenumber Transformation Method in Near Surface Stratigraphic Structure Detection [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2020, 56(3): 427-435.
[8]	YE Nini, LU Keding, DONG Huabin, WU Yusheng, ZENG Limin, ZHANG Yuanhang. A Study of the Water-Soluble Inorganic Salts and Their Gaseous Precursors at Wangdu Site in the Summer Time [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2016, 52(6): 1109-1117.
[9]	DAI Youshan,DAI Liang. Universal Mass-Velocity Relation and Special Relativity [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2014, 50(3): 403-410.
[10]	CAI Cai,LI Peijun,GUO Jiancong. Segmentation of High Resolution Imagery over Urban Area Using Watershed Transformation and Stratified Region Merging [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2014, 50(2): 323-330.
[11]	ZUO Zhiquan,LIU Zhengjun,ZHANG Li. Theoretical Model of Non-linear ICP Co-registration Based on Fast Approximation of 1st Polynomials Extension [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2013, 49(5): 867-872.
[12]	DAI Youshan. Special Relativity and Upper Limit for Speed [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2013, 49(3): 356-364.
[13]	WEI Jianping. Wave Propagationin Elastic Square Columns Ⅳ. Effect of Boundary Condition [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2009, 45(5): 743-747.
[14]	XU Ying,Lv Bin. Application of Land Transformation Model Based on GIS and ANN:A Case Study of Beijing, China [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2008, 44(2): 262-270.
[15]	CHEN Fang,NIU Zheng,FU Anmin. Study on Lower Spatial Resolution Image Simulation of Field Survey Data [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2006, 42(4): 478-483.

Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae

蓝藻光合基因psaC在酵母中的转化和表达

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics