In order to study the expression and localization of S100A4 and S100A10 at different time points of skin hair follicle development in C57BL/6N mice, E13.5, E15.5, E18.5 pregnant mouse embryos and P2, P4, P8 newborn mice were selected. Three mice were taken at each time-point. The expression and localization of S100A4 and S100A10 mRNA and proteins in the back skin of mice were detected by molecular biology and histological techniques. The results showed that the mRNA and protein expressions of S100A4 and S100A10 firstly increased and then decreased during the development of hair follicles, and the mRNA and protein expressions were the highest at 2 days after birth. Immunohistochemical localization showed that S100A4 protein and S100A10 protein were continuously expressed in mouse hair follicle development, but their localization regions were different. It is suggested that S100A4 and S100A10 may play an important role in the development of mouse hair follicles. In conclusion, the expression of S100A4 and S100A10 is different in the back skin of mice at different time points of hair follicle development, and S100A4 and S100A10 participate in the development process of mouse hair follicles. 

为了研究S100A4和S100A10在C57BL/6N小鼠皮肤毛囊发育不同时间点的表达与定位, 选取E13.5, E15.5, E18.5孕鼠胚胎和新生鼠P2, P4, P8小鼠, 每个发育时间点各取3只小鼠, 取背部皮肤组织, 通过分子生物学及组织学技术, 检测不同时期小鼠背部皮肤中S100A4和S100A10 mRNA及蛋白的表达与定位。结果表明, 在毛囊发育时期, S100A4和S100A10的mRNA和蛋白表达均先升高后降低, 出生2天时的mRNA和蛋白表达最高。免疫组织化学定位显示, S100A4蛋白和S100A10蛋白在小鼠毛囊发育中持续表达, 但是定位区域有所不同, 提示S100A4和S100A10可能在小鼠毛囊发育过程中具有重要的作用。综上所述, S100A4和S100A10在小鼠毛囊发育不同时间点的背部皮肤中表达不同, S100A4和S100A10参与小鼠毛囊发育过程。