Abstract: This paper introduces the fabrication of microfluidic chips based on integrating the agarose structure and their application to bacterial culture. Two designs of microfluidic chips were devised. One was a chemostat in microfluidic chip， applied to the killing kinetics of cells. The microfluidic chip showed several advantages， measuring the data on-line， simplify the experimental manipulation and feasible output of tolerant cells. Therefore it holds potential for research of the killing kinetics of cells. The other had three different high micro-culture chambers and the bacterial population was measured by two ways-fluorescent intensity and OD value， by which more information about the bacterial population was acquired. The experiments indicated that the measuring tendency of bacterial growth by OD value was consistent with one by fluorescent intensity. There are other advantages in such microfluidic chip that it is easy to fabricate the microfluidic chips and the agarose confines bacteria in the chambers and cell nutrients are diffusible in agarose， which offer a good means to research the motile bacteria.

Key words: microfluidic, agarose, bacteria, OD value, metapopulations, chemostat

摘要： 介绍集成琼脂糖结构的微流芯片的制作过程及其在菌群培养中的应用。设计了两种集成琼脂糖结构的微流芯片。第一种结构的芯片是恒化器（chemostat）微流芯片，可以用于研究杀菌动力学。这种微流芯片具有实现实时检测，简化实验操作，可以输出耐药菌等优点，有潜力成为研究杀菌动力学的一种方法。为了获得与细菌数目有关的更多信息，第二种结构的芯片集成3个不同高度的培养室，采用测量荧光强度和光密度值（optical density value, OD value）的方法来表征不同高度培养室的细菌数目。结果表明，这两种方法测量得到的细菌生长趋势基本上是一致的。这两种结构的芯片具有易于加工，限制细菌在培养室内生长而且允许营养物质自由扩散等优点，为研究可游动细菌提供了一个简便的方法。

关键词: 微流, 琼脂糖, 细菌, 光密度值, 种群, 恒化器