Abstract: Terminal restriction fragment length polymorphism (TRFLP) was used to investigate the change of bacterial and archaeal communities in leachate-contaminated aquifer in response to anaerobic phenanthrene biodegradation. Results show that a great change in bacterial community occurred with phenanthrene biodegradation. The ribotype and Shannon-Weiner index increase from 15 to 23, and 2.39 to 2.88 respectively. However, archaeal community only shows an insignificant change with phenanthrene biodegradation, and ribotype and Shannon-Weiner index vary slightly.

Key words: polycyclic aromatic hydrocarbons (PAHs), biodegradation, microbial community, terminal restriction fragment length polymorphism (TRFLP)

摘要： 利用TRFLP技术研究了受垃圾渗滤液污染的地下沉积物中细菌和古细菌群落在菲厌氧降解前后的变化。结果表明: 细菌群落在生物降解过程中变化很大, 物种丰度及Shannon-Weiner指数分别由15和2.39增加到23和2.88; 古细菌群落在生物降解过程中变化较小, 物种丰度及Shannon-Weiner指数变化不大。

关键词: 多环芳烃, 生物降解, 微生物群落, TRFLP技术